Molecular characterization and evolutionary analysis of soybean mosaic virus infecting Pinellia ternata in China

• Published in: Virus genes 2008-02, Vol.36 (1), p.177-190
• Main Authors: Sun, Haohua, ShenTu, Susu, Xue, Feng, Duns, Greg, Chen, Jishuang
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Biomedical and Life Sciences; Biomedicine; China; Citrullus lanatus; Dasheen mosaic virus; Evolution, Molecular; Genome, Viral; Glycine max; Medical Microbiology; Molecular Sequence Data; Mosaic Viruses - genetics; Mosaic Viruses - immunology; Mosaic Viruses - isolation & purification; Phylogeny; Pinellia - virology; Pinellia ternata; Plant Diseases - virology; Plant Leaves - virology; Plant Sciences; Potyvirus; RNA, Viral; Sequence Homology, Nucleic Acid; Soybean mosaic virus; Virology
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-007-0167-z

Twenty-nine Pinellia ternata specimens were collected from representative areas in China, including the major production provinces of Zhejiang, Henan, Shanxi, Hunan, Shandong and Hubei. Seven isolates related to soybean mosaic virus (SMV), which could be pathogenic on P. ternata and some soybean [Glycine max (L.) Merr.] cultivars, were detected using double antibody sandwich immunosorbent assay (DAS-ELISA) and RT-PCR amplification performed with degenerate primer of potyviruses. It is revealed that the common potyvirus infecting P. ternata is, indeed, only SMVs rather than Dasheen mosaic virus (DsMV) as previously reported. Further molecular phylogenetic analysis of the coat protein (CP) genes of these SMV isolates from P. ternata and G. max, along with some other potyvirus members, such as DsMV and Watermelon mosaic virus (WMV) reconstructed the evolutionary route on both nucleotide and amino acid levels. Similarity and homology of nucleotide sequences for SMV CP genes demonstrated high host correlation and low partial habitat correlation, while those of amino acid sequences also showed that the host correlation was more notable than the habitat correlation. The amino acid sequence of conserved region within CP determines the main function, which shows high homology between species. This study outspreaded from the viruses themselves and their relationship to the infected hosts and revealed the evolutionary strategies, especially the rapid variation or recombination of SMV of P. ternata, in order to adapt itself naturally to the special host.