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Prevention of zona hardening in non-human primate oocytes cultured in protein-free medium
Background Development of a protein‐free medium for in vitro maturation of oocytes that prevents zona hardening is essential for the study of components that affect the maturation process. Methods Immature macaque oocytes were cultured in modified CMRL medium with serum protein or without protein [w...
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| Published in: | Journal of medical primatology 2007-02, Vol.36 (1), p.10-16 |
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| container_end_page | 16 |
| container_issue | 1 |
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| container_title | Journal of medical primatology |
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| creator | VandeVoort, Catherine A. Hung, Pei-hsuan Schramm, R.D. |
| description | Background Development of a protein‐free medium for in vitro maturation of oocytes that prevents zona hardening is essential for the study of components that affect the maturation process.
Methods Immature macaque oocytes were cultured in modified CMRL medium with serum protein or without protein [with or without polyvinyl alcohol (PVA)] for 24 hours.
Results Sperm penetration of oocytes cultured for 24 hours prior to insemination was poor in CMRL medium alone, but was dramatically improved in CMRL medium with the addition of either PVA or BCS. In the second experiment, in vivo matured oocytes were cultured in CMRL with PVA or serum for 6 hours prior to insemination. The incidence of fertilization and embryo development to the blastocyst stage were similar in CMRL with PVA.
Conclusions These results indicate that fertilization failure occurs when macaque oocytes are cultured in medium without protein, but can be prevented with PVA. |
| doi_str_mv | 10.1111/j.1600-0684.2006.00206.x |
| format | article |
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Methods Immature macaque oocytes were cultured in modified CMRL medium with serum protein or without protein [with or without polyvinyl alcohol (PVA)] for 24 hours.
Results Sperm penetration of oocytes cultured for 24 hours prior to insemination was poor in CMRL medium alone, but was dramatically improved in CMRL medium with the addition of either PVA or BCS. In the second experiment, in vivo matured oocytes were cultured in CMRL with PVA or serum for 6 hours prior to insemination. The incidence of fertilization and embryo development to the blastocyst stage were similar in CMRL with PVA.
Conclusions These results indicate that fertilization failure occurs when macaque oocytes are cultured in medium without protein, but can be prevented with PVA.</description><identifier>ISSN: 0047-2565</identifier><identifier>EISSN: 1600-0684</identifier><identifier>DOI: 10.1111/j.1600-0684.2006.00206.x</identifier><identifier>PMID: 17359460</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Cells, Cultured ; Chemically defined culture media ; Culture Media - chemistry ; embryo development ; Female ; fertilization ; Fertilization in Vitro ; in vitro maturation ; Macaca mulatta - physiology ; Male ; Proteins - chemistry ; Sperm-Ovum Interactions - physiology ; Spermatozoa - physiology ; Zona Pellucida - physiology</subject><ispartof>Journal of medical primatology, 2007-02, Vol.36 (1), p.10-16</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4046-60109c47b7528c6f2c1f45817cbcbeb19b811c428e7fd3810d0cd7855f92344f3</citedby><cites>FETCH-LOGICAL-c4046-60109c47b7528c6f2c1f45817cbcbeb19b811c428e7fd3810d0cd7855f92344f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17359460$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VandeVoort, Catherine A.</creatorcontrib><creatorcontrib>Hung, Pei-hsuan</creatorcontrib><creatorcontrib>Schramm, R.D.</creatorcontrib><title>Prevention of zona hardening in non-human primate oocytes cultured in protein-free medium</title><title>Journal of medical primatology</title><addtitle>J Med Primatol</addtitle><description>Background Development of a protein‐free medium for in vitro maturation of oocytes that prevents zona hardening is essential for the study of components that affect the maturation process.
Methods Immature macaque oocytes were cultured in modified CMRL medium with serum protein or without protein [with or without polyvinyl alcohol (PVA)] for 24 hours.
Results Sperm penetration of oocytes cultured for 24 hours prior to insemination was poor in CMRL medium alone, but was dramatically improved in CMRL medium with the addition of either PVA or BCS. In the second experiment, in vivo matured oocytes were cultured in CMRL with PVA or serum for 6 hours prior to insemination. The incidence of fertilization and embryo development to the blastocyst stage were similar in CMRL with PVA.
Conclusions These results indicate that fertilization failure occurs when macaque oocytes are cultured in medium without protein, but can be prevented with PVA.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chemically defined culture media</subject><subject>Culture Media - chemistry</subject><subject>embryo development</subject><subject>Female</subject><subject>fertilization</subject><subject>Fertilization in Vitro</subject><subject>in vitro maturation</subject><subject>Macaca mulatta - physiology</subject><subject>Male</subject><subject>Proteins - chemistry</subject><subject>Sperm-Ovum Interactions - physiology</subject><subject>Spermatozoa - physiology</subject><subject>Zona Pellucida - physiology</subject><issn>0047-2565</issn><issn>1600-0684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNkE1v1DAQhi0EokvhLyCfuGUZJ_6KxAVVsBS1UFUgxMlKnAn1ktjFTuguvx6nuypXfLAt-XlnPA8hlMGa5fV6u2YSoACp-boEkGuAMu-7R2T18PCYrAC4KkohxQl5ltIWACpe86fkhKlK1FzCiny_ivgb_eSCp6Gnf4Jv6E0TO_TO_6DOUx98cTOPjae30Y3NhDQEu58wUTsP0xyxW6jbGCZ0vugjIh2xc_P4nDzpmyHhi-N5Sr6-f_fl7ENx8Xlzfvb2orAcuCwkMKgtV60SpbayLy3rudBM2da22LK61YxZXmpUfVdpBh3YTmkh-rqsOO-rU_LqUDf_4deMaTKjSxaHofEY5mQUlFIwDhnUB9DGkFLE3txPFPeGgVm0mq1Z7JnFnlm0mnutZpejL4895jZP9y949JiBNwfgzg24_-_C5uPlVb7keHGIuzTh7iHexJ9GqkoJ8-3TxtQbBfr6-tLU1V8WzpUc</recordid><startdate>200702</startdate><enddate>200702</enddate><creator>VandeVoort, Catherine A.</creator><creator>Hung, Pei-hsuan</creator><creator>Schramm, R.D.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200702</creationdate><title>Prevention of zona hardening in non-human primate oocytes cultured in protein-free medium</title><author>VandeVoort, Catherine A. ; Hung, Pei-hsuan ; Schramm, R.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4046-60109c47b7528c6f2c1f45817cbcbeb19b811c428e7fd3810d0cd7855f92344f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chemically defined culture media</topic><topic>Culture Media - chemistry</topic><topic>embryo development</topic><topic>Female</topic><topic>fertilization</topic><topic>Fertilization in Vitro</topic><topic>in vitro maturation</topic><topic>Macaca mulatta - physiology</topic><topic>Male</topic><topic>Proteins - chemistry</topic><topic>Sperm-Ovum Interactions - physiology</topic><topic>Spermatozoa - physiology</topic><topic>Zona Pellucida - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VandeVoort, Catherine A.</creatorcontrib><creatorcontrib>Hung, Pei-hsuan</creatorcontrib><creatorcontrib>Schramm, R.D.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical primatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>VandeVoort, Catherine A.</au><au>Hung, Pei-hsuan</au><au>Schramm, R.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevention of zona hardening in non-human primate oocytes cultured in protein-free medium</atitle><jtitle>Journal of medical primatology</jtitle><addtitle>J Med Primatol</addtitle><date>2007-02</date><risdate>2007</risdate><volume>36</volume><issue>1</issue><spage>10</spage><epage>16</epage><pages>10-16</pages><issn>0047-2565</issn><eissn>1600-0684</eissn><abstract>Background Development of a protein‐free medium for in vitro maturation of oocytes that prevents zona hardening is essential for the study of components that affect the maturation process.
Methods Immature macaque oocytes were cultured in modified CMRL medium with serum protein or without protein [with or without polyvinyl alcohol (PVA)] for 24 hours.
Results Sperm penetration of oocytes cultured for 24 hours prior to insemination was poor in CMRL medium alone, but was dramatically improved in CMRL medium with the addition of either PVA or BCS. In the second experiment, in vivo matured oocytes were cultured in CMRL with PVA or serum for 6 hours prior to insemination. The incidence of fertilization and embryo development to the blastocyst stage were similar in CMRL with PVA.
Conclusions These results indicate that fertilization failure occurs when macaque oocytes are cultured in medium without protein, but can be prevented with PVA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17359460</pmid><doi>10.1111/j.1600-0684.2006.00206.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of medical primatology, 2007-02, Vol.36 (1), p.10-16 |
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| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Animals Cells, Cultured Chemically defined culture media Culture Media - chemistry embryo development Female fertilization Fertilization in Vitro in vitro maturation Macaca mulatta - physiology Male Proteins - chemistry Sperm-Ovum Interactions - physiology Spermatozoa - physiology Zona Pellucida - physiology |
| title | Prevention of zona hardening in non-human primate oocytes cultured in protein-free medium |
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