In vitro analysis of the TAT protein transduction domain as a drug delivery vehicle in protozoan parasites

The present study was undertaken to analyse the capability of HIV-1 derived TAT protein transduction domain (PTD) fused with Green Fluorescent Protein (TAT-GFP) as a delivery vehicle into a range of protozoan parasites. Successful transduction of native purified TAT-GFP was observed by fluorescent m...

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Bibliographic Details
Published in:Experimental parasitology 2008-03, Vol.118 (3), p.303-307
Main Authors: Chin Lee, Stephanie Hui, Jefferies, Ryan, Watt, Paul, Hopkins, Richard, Sotzik, Frank, Reid, Simon, Armson, Anthony, Boxell, Annika, Ryan, Una
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cats
Cryptosporidium
Cryptosporidium parvum
Cryptosporidium parvum - metabolism
drug delivery systems
Drug Delivery Systems - methods
Drug delivery vehicle
Fundamental and applied biological sciences. Psychology
Giardia
Giardia - metabolism
Giardia duodenalis
Giardia lamblia
green fluorescent protein
Green Fluorescent Proteins - physiology
Human immunodeficiency virus 1
Image Processing, Computer-Assisted
Life cycle. Host-agent relationship. Pathogenesis
Mice
Microscopy, Fluorescence
Neospora
Neospora - metabolism
Neospora caninum
peptide delivery
peptide transduction
peptides
Protein transduction domain (PTD)
Protozoa
protozoal infections
recombinant fusion proteins
tat Gene Products, Human Immunodeficiency Virus - physiology
TAT-GFP
Toxoplasma - metabolism
uptake mechanisms
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Summary:The present study was undertaken to analyse the capability of HIV-1 derived TAT protein transduction domain (PTD) fused with Green Fluorescent Protein (TAT-GFP) as a delivery vehicle into a range of protozoan parasites. Successful transduction of native purified TAT-GFP was observed by fluorescent microscopy in Cryptosporidium parvum, Giardia duodenalis, and Neospora caninum. The ability to transduce peptides and other cargo into protozoan parasites, will greatly assist in the delivery of future peptide-based drugs and target validation peptides.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2007.08.019