Preparation of Glucosamine from Exoskeleton of Shrimp and Predicting Production Yield by Response Surface Methodology

• Published in: Journal of agricultural and food chemistry 2007-03, Vol.55 (6), p.2246-2250
• Main Authors: Mojarrad, Javid S, Nemati, Mahboob, Valizadeh, Hadi, Ansarin, Masood, Bourbour, Samira
• Format: Article
• Language: English
• Subjects: acid treatment; Animals; Biological and medical sciences; chitin; Chitin - chemistry; Decapoda; exoskeleton; Fish and seafood industries; Food industries; Fundamental and applied biological sciences. Psychology; glucosamine; Glucosamine - chemistry; hydrochloric acid; Hydrochloric Acid - chemistry; Hydrolysis; Metapenaeus monoceros; Penaeidae - chemistry; response surface methodology; Solutions
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf062983a

Chitin was prepared from Persian Gulf shrimp (Metapenaeus monoceros), and then, the obtained chitin was hydrolyzed by hydrochloric acid solutions. The production yield of glucosamine hydrochloride from chitin was optimized, and the effect of three factors (acid concentration, acid to chitin ratio, and reaction time) was investigated. A Box−Behnken design by Minitab software created 12 reactions with different conditions. Each reaction was performed in two replicates. Response surface methodology was used for predicting the glucosamine preparation. The optimum conditions for glucosamine hydrochloride preparation were 30 and 37% hydrochloric acid, 9:1 (v/w) acid solution to solid ratio, and 4 h of reaction time. Time ratio and time acid concentrations were the effective factors on the yield. Keywords: Chitin; glucosamine; hydrolysis; response surface methodology