Plasma membrane Ca2+-ATPase expression during colon cancer cell line differentiation

The differentiation of colon cancer cell lines is associated with changes in calcium homeostasis. Concomitantly there are changes in the expression of some calcium transporters and G-protein-coupled receptors, which are capable of altering cytosolic-free calcium levels. Recent studies associate alte...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2007-04, Vol.355 (4), p.932-936
Main Authors: Aung, Cho S., Kruger, Wade A., Poronnik, Philip, Roberts-Thomson, Sarah J., Monteith, Gregory R.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Butyric Acid - pharmacology
Calcium
Calcium ATPase
Calcium-Transporting ATPases - genetics
Calcium-Transporting ATPases - metabolism
Cancer
Cell Differentiation - drug effects
Cell Line, Tumor
Cell Membrane - enzymology
Colon cancer
Colonic Neoplasms - enzymology
Colonic Neoplasms - genetics
Colonic Neoplasms - pathology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
mRNA
PMCA
Protein
RNA, Messenger - genetics
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Summary:The differentiation of colon cancer cell lines is associated with changes in calcium homeostasis. Concomitantly there are changes in the expression of some calcium transporters and G-protein-coupled receptors, which are capable of altering cytosolic-free calcium levels. Recent studies associate alterations in calcium transporter expression with tumourigenesis, such as changes in specific isoforms of the plasma membrane calcium ATPase (PMCA) in breast cancer cell lines. In this study, we examined the expression of PMCA isoforms in the HT-29 colon cancer cell line using two methods of differentiation (sodium butyrate-mediated and spontaneous post-confluency induced differentiation). Our studies show that differentiation of HT-29 colon cancer cells is associated with the up-regulation of the PMCA isoform PMCA4 but no significant alteration in PMCA1. These results suggest that PMCA4 may be important and have a specific role in colon cells as well as being significant in colon cancer tumourigenesis.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2007.02.050