Tissue microarray - a high-throughput molecular analysis in head and neck cancer

• Published in: Journal of oral pathology & medicine 2008-03, Vol.37 (3), p.166-176
• Main Authors: Radhakrishnan, Raghu, Solomon, Monica, Satyamoorthy, Kapaettu, Martin, Leslie E., Lingen, Mark W.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biomarkers, Tumor - analysis; Carcinoma, Squamous Cell - chemistry; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Dentistry; Gene Expression Profiling; head and neck cancer; Head and Neck Neoplasms - chemistry; Head and Neck Neoplasms - genetics; Head and Neck Neoplasms - pathology; Humans; Kaplan-Meier Estimate; Medical sciences; oral cancer; Otorhinolaryngology (head neck, general aspects and miscellaneous); Otorhinolaryngology. Stomatology; Proportional Hazards Models; Signal Transduction; Tissue Array Analysis; tissue microarray; Tumors; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1111/j.1600-0714.2007.00606.x

With the identification of a number of novel markers having diagnostic, prognostic, and therapeutic significance, the application of tissue microarray (TMA) has become a valuable tool for validating candidate markers in cancer research. The TMA is a high‐throughput technique, which allows large‐scale analyses of hundreds of archival clinical tissue samples using the ‘array’ approach. This paper highlights briefly its robust technology, technical aspects of its construction, and the validity of the TMA results for oral pathology diagnostics by reviewing data from recent literature particularly with reference to head and neck cancer.