Novel Phosphorylcholine-Containing Protein of Pseudomonas aeruginosa Chronic Infection Isolates Interacts with Airway Epithelial Cells

Pseudomonas aeruginosa undergoes phase variation in the expression of the phosphorylcholine (ChoP) epitope, a structure crucial for the virulence of several respiratory pathogens. In this study, ChoP expression analysis comparing organisms from acute and chronic infections revealed that expression o...

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Bibliographic Details
Published in:The Journal of infectious diseases 2008-02, Vol.197 (3), p.465-473
Main Authors: Barbier, Mariette, Oliver, Antonio, Rao, Jayasimha, Hanna, Sheri L., Goldberg, Joanna B., Albertí, Sebastián
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Antibodies
Bacteria
Bacterial Proteins - pharmacology
Bacteriology
Biological and medical sciences
Chronic Disease
Cystic fibrosis
Disease Models, Animal
Epithelial cells
Epitopes
Fundamental and applied biological sciences. Psychology
Gels
Humans
Infections
Lungs
Mice
Mice, Inbred ICR
Microbiology
Miscellaneous
Monoclonal antibodies
Pathogens
Phosphorylcholine - pharmacology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - isolation & purification
Pseudomonas aeruginosa - pathogenicity
Pseudomonas Infections - physiopathology
Respiratory Mucosa - drug effects
Respiratory Mucosa - microbiology
Sputum - microbiology
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Summary:Pseudomonas aeruginosa undergoes phase variation in the expression of the phosphorylcholine (ChoP) epitope, a structure crucial for the virulence of several respiratory pathogens. In this study, ChoP expression analysis comparing organisms from acute and chronic infections revealed that expression of ChoP at 37°C was higher among strains from chronic infections. Coimmunoprecipitation experiments and mass spectrometry analysis demonstrated that ChoP was on the protein elongation factor Tu. The presence of ChoP at the surface was confirmed by immunofluorescence and flow cytometry analysis of intact bacteria. Pretreatment of bronchial epithelial cells or mice with a platelet-activating factor receptor (PAFR) antagonist reduced adhesion and invasion of the ChoP-positive P. aeruginosa isolates. Results of this study suggest that ChoP expression may represent a novel phenotype expressed by the chronic infection isolates that could mediate P. aeruginosa colonization of the epithelial airway by means of the interaction with the PAFR.
ISSN:0022-1899
1537-6613
DOI:10.1086/525048