Comparison of high performance TLC and HPLC for separation and quantification of chlorogenic acid in green coffee bean extracts

Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water...

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Bibliographic Details
Published in:Journal of separation science 2008-02, Vol.31 (2), p.237-241
Main Authors: Urakova, Irina N, Pozharitskaya, Olga N, Shikov, Alexander N, Kosman, Vera M, Makarov, Valery G
Format: Article
Language:English
Subjects:
Chlorogenic acid
Chlorogenic Acid - analysis
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer - methods
Coffee - chemistry
Green coffee bean extracts
High performance TLC
HPLC
Plant Extracts - chemistry
Quantitative determination
Spectrophotometry, Ultraviolet
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Summary:Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts. For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used. Densitometric determination of chlorogenic acid by HPTLC was performed at 330 nm. A gradient RP HPLC method was carried out at 330 nm. All necessary validation tests for both methods were developed for their comparison. There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200700472