Vacuolar H+-ATPase expression is increased in acid-secreting intercalated cells in kidneys of rats with hypercalcaemia-induced alkalosis

• Published in: Acta Physiologica 2007-04, Vol.189 (4), p.359-368
• Main Authors: Wang, W., Praetorius, J., Li, C., Praetorius, H. A., Kwon, T.-H., Frøkiær, J., Nielsen, S.
• Format: Article
• Language: English
• Subjects: Alkalosis - blood; Alkalosis - metabolism; Animals; Anion Exchange Protein 1, Erythrocyte - analysis; anion exchanger AE2; Anion Transport Proteins - analysis; Antiporters - analysis; Biological and medical sciences; Chloride-Bicarbonate Antiporters - analysis; electroneutral NBCn1; Fundamental and applied biological sciences. Psychology; H+-ATPase; Hypercalcemia - blood; Hypercalcemia - metabolism; Immunohistochemistry - methods; Infusions, Parenteral; kidney; Kidney - enzymology; Kidney - metabolism; Kidney Cortex - enzymology; Kidney Cortex - metabolism; Kidney Medulla - enzymology; Kidney Medulla - metabolism; Male; metabolic alkalosis; Parathyroid Hormone - administration & dosage; Proton-Translocating ATPases - analysis; Rats; Rats, Wistar; SLC4A Proteins; Sodium-Bicarbonate Symporters - analysis; Vacuoles - enzymology; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 1748-1708; 1748-1716
• DOI: 10.1111/j.1748-1716.2007.01672.x

Aims:  Hypercalcaemia is known to be associated with systemic metabolic alkalosis, although the underlying mechanism is uncertain. Therefore, we aimed to examine whether hypercalcaemia was associated with changes in the expression of acid–base transporters in the kidney. Methods:  Rats were infused with human parathyroid hormone (PTH, 15 μg kg−1 day−1), or vehicle for 48 h using osmotic minipumps. Results:  The rats treated with PTH developed hypercalcaemia and exhibited metabolic alkalosis (arterial HCO: 31.1 ± 0.8 vs. 28.1 ± 0.8 mmol L−1 in controls, P