Microarray analysis reveals influence of the sesquiterpene lactone parthenolide on gene transcription profiles in human epithelial cells

• Published in: Life sciences (1973) 2007-04, Vol.80 (17), p.1608-1618
• Main Authors: Lindenmeyer, M.T., Kern, C., Sparna, T., Donauer, J., Wilpert, J., Schwager, J., Porath, D., Kreutz, C., Timmer, J., Merfort, I.
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents, Non-Steroidal - pharmacology; cDNA microarray; Cell Line; Dose-Response Relationship, Drug; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Gene Expression Profiling; Gene Expression Regulation - drug effects; Gene transcription; Humans; Oligonucleotide Array Sequence Analysis; Parthenolide; Real-time quantitative PCR; RNA, Messenger - metabolism; Sesquiterpene lactones; Sesquiterpenes - pharmacology; Transcription, Genetic - drug effects; Tumor Necrosis Factor-alpha - pharmacology
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2007.01.036

Sesquiterpene lactones are known for their anti-inflammatory activity which has been proven in various assays on DNA, mRNA and protein level. Here we report on the change in the gene expression profile in TNF-α stimulated human 293 cells after treatment with parthenolide using a cDNA microarray analysis. Twenty-one of 7028 genes were found to be up- and 18 down-regulated. They encode for chemoattractants, immune system proteins, glycoproteins, metabolism, serine proteinases, and transcription factors. Confirmatory analyses were carried out using quantitative real-time RT-PCR (TaqMan ®). Additional studies with selected genes revealed the concentration-dependent influence of parthenolide on the expression of these genes.