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Permeability of the reconstructed human epidermis model Episkin ® in comparison to various human skin preparations
The objective of this work was to compare the barrier function of the small diameter reconstructed human epidermis model Episkin ® ( d = 12 mm) to human skin in vitro. For that purpose a modification for the Franz diffusion cell ( d = 15 mm) had to be developed so as to allow direct comparison with...
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Published in: | European journal of pharmaceutics and biopharmaceutics 2007-04, Vol.66 (1), p.127-134 |
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container_title | European journal of pharmaceutics and biopharmaceutics |
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creator | Netzlaff, Frank Kaca, Monika Bock, Udo Haltner-Ukomadu, Eleonore Meiers, Peter Lehr, Claus-Michael Schaefer, Ulrich F. |
description | The objective of this work was to compare the barrier function of the small diameter reconstructed human epidermis model Episkin
® (
d
=
12
mm) to human skin
in vitro. For that purpose a modification for the Franz diffusion cell (
d
=
15
mm) had to be developed so as to allow direct comparison with the following human skin preparations: Full thickness skin (FTS), split thickness skin (STS), heat-separated epidermis (HSE), and trypsin isolated stratum corneum (TISC). Among the tested preparations, HSE appeared to be the most preferable due to its clear morphological structure and ease of preparation. The lipid profile of HSE and Episkin
® was analyzed and showed significant differences in terms of cholesterol, ceramides and triglycerides contents, whereas cholesterol esters and fatty acids were not different. Permeation data with HSE and Episkin
® were then gathered using caffeine and testosterone. Both test compounds permeated much faster through Episkin
® than through HSE. Moreover, opposed to Episkin
®, HSE differentiated between the two test compounds. In spite of the remarkable progress in developing RHEs in the past years at this time Episkin
® can obviously not yet fully replace human skin for
in vitro permeability experiments. |
doi_str_mv | 10.1016/j.ejpb.2006.08.012 |
format | article |
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® (
d
=
12
mm) to human skin
in vitro. For that purpose a modification for the Franz diffusion cell (
d
=
15
mm) had to be developed so as to allow direct comparison with the following human skin preparations: Full thickness skin (FTS), split thickness skin (STS), heat-separated epidermis (HSE), and trypsin isolated stratum corneum (TISC). Among the tested preparations, HSE appeared to be the most preferable due to its clear morphological structure and ease of preparation. The lipid profile of HSE and Episkin
® was analyzed and showed significant differences in terms of cholesterol, ceramides and triglycerides contents, whereas cholesterol esters and fatty acids were not different. Permeation data with HSE and Episkin
® were then gathered using caffeine and testosterone. Both test compounds permeated much faster through Episkin
® than through HSE. Moreover, opposed to Episkin
®, HSE differentiated between the two test compounds. In spite of the remarkable progress in developing RHEs in the past years at this time Episkin
® can obviously not yet fully replace human skin for
in vitro permeability experiments.</description><identifier>ISSN: 0939-6411</identifier><identifier>EISSN: 1873-3441</identifier><identifier>DOI: 10.1016/j.ejpb.2006.08.012</identifier><identifier>PMID: 17029766</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Caffeine ; Ceramides - analysis ; Cholesterol Esters - analysis ; Chromatography, High Pressure Liquid ; Collagen - analysis ; Epidermis - anatomy & histology ; Epidermis - chemistry ; Epidermis - physiology ; Episkin ; Fatty Acids - analysis ; Female ; Full thickness skin ; General pharmacology ; Heat-separated human epidermis ; Hot Temperature ; Humans ; Medical sciences ; Permeability ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Skin - anatomy & histology ; Skin - chemistry ; Skin lipids ; Skin, Artificial ; Split thickness skin ; Stratum corneum ; Testosterone ; Tissue Engineering - methods ; Triglycerides - analysis</subject><ispartof>European journal of pharmaceutics and biopharmaceutics, 2007-04, Vol.66 (1), p.127-134</ispartof><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-1723616500bbf7b36007a0d75529dd69e665ca51d5ff61524d157cd0a1f0803d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18638832$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17029766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Netzlaff, Frank</creatorcontrib><creatorcontrib>Kaca, Monika</creatorcontrib><creatorcontrib>Bock, Udo</creatorcontrib><creatorcontrib>Haltner-Ukomadu, Eleonore</creatorcontrib><creatorcontrib>Meiers, Peter</creatorcontrib><creatorcontrib>Lehr, Claus-Michael</creatorcontrib><creatorcontrib>Schaefer, Ulrich F.</creatorcontrib><title>Permeability of the reconstructed human epidermis model Episkin ® in comparison to various human skin preparations</title><title>European journal of pharmaceutics and biopharmaceutics</title><addtitle>Eur J Pharm Biopharm</addtitle><description>The objective of this work was to compare the barrier function of the small diameter reconstructed human epidermis model Episkin
® (
d
=
12
mm) to human skin
in vitro. For that purpose a modification for the Franz diffusion cell (
d
=
15
mm) had to be developed so as to allow direct comparison with the following human skin preparations: Full thickness skin (FTS), split thickness skin (STS), heat-separated epidermis (HSE), and trypsin isolated stratum corneum (TISC). Among the tested preparations, HSE appeared to be the most preferable due to its clear morphological structure and ease of preparation. The lipid profile of HSE and Episkin
® was analyzed and showed significant differences in terms of cholesterol, ceramides and triglycerides contents, whereas cholesterol esters and fatty acids were not different. Permeation data with HSE and Episkin
® were then gathered using caffeine and testosterone. Both test compounds permeated much faster through Episkin
® than through HSE. Moreover, opposed to Episkin
®, HSE differentiated between the two test compounds. In spite of the remarkable progress in developing RHEs in the past years at this time Episkin
® can obviously not yet fully replace human skin for
in vitro permeability experiments.</description><subject>Biological and medical sciences</subject><subject>Caffeine</subject><subject>Ceramides - analysis</subject><subject>Cholesterol Esters - analysis</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Collagen - analysis</subject><subject>Epidermis - anatomy & histology</subject><subject>Epidermis - chemistry</subject><subject>Epidermis - physiology</subject><subject>Episkin</subject><subject>Fatty Acids - analysis</subject><subject>Female</subject><subject>Full thickness skin</subject><subject>General pharmacology</subject><subject>Heat-separated human epidermis</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Permeability</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Skin - anatomy & histology</subject><subject>Skin - chemistry</subject><subject>Skin lipids</subject><subject>Skin, Artificial</subject><subject>Split thickness skin</subject><subject>Stratum corneum</subject><subject>Testosterone</subject><subject>Tissue Engineering - methods</subject><subject>Triglycerides - analysis</subject><issn>0939-6411</issn><issn>1873-3441</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kc2O1SAYhonROMfRG3Bh2Oiu9aO0QBM3ZjL-JJPoQteEwtcMx7ZUoJPMTXkRc2VyPE1m5wZYPO-bNw-EvGZQM2Di_bHG4zrUDYCoQdXAmifkwJTkFW9b9pQcoOd9JVrGLsiLlI4A0MpOPScXTELTSyEOJH3HOKMZ_OTzPQ0jzbdII9qwpBw3m9HR2202C8XVu4L6ROfgcKLXq0-__EIf_tBy2jCvJvoUFpoDvSvPsKU9-Q9bIxbAZF-KX5Jno5kSvtrvS_Lz0_WPqy_VzbfPX68-3lS2zMwVkw0XTHQAwzDKgQsAacDJrmt650SPQnTWdMx14yhY17SOddI6MGwEBdzxS_Lu3LvG8HvDlHWZb3GazIJlni4SFOesL2BzBm0MKUUc9Rr9bOK9ZqBPqvVRn1Trk2oNShfVJfRmb9-GGd1jZHdbgLc7YJI10xjNYn165JTgSvFT0Yczh8XFnceok_W4WHS-fETWLvj_7fgLv92evg</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Netzlaff, Frank</creator><creator>Kaca, Monika</creator><creator>Bock, Udo</creator><creator>Haltner-Ukomadu, Eleonore</creator><creator>Meiers, Peter</creator><creator>Lehr, Claus-Michael</creator><creator>Schaefer, Ulrich F.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Permeability of the reconstructed human epidermis model Episkin ® in comparison to various human skin preparations</title><author>Netzlaff, Frank ; Kaca, Monika ; Bock, Udo ; Haltner-Ukomadu, Eleonore ; Meiers, Peter ; Lehr, Claus-Michael ; Schaefer, Ulrich F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-1723616500bbf7b36007a0d75529dd69e665ca51d5ff61524d157cd0a1f0803d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Caffeine</topic><topic>Ceramides - analysis</topic><topic>Cholesterol Esters - analysis</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Collagen - analysis</topic><topic>Epidermis - anatomy & histology</topic><topic>Epidermis - chemistry</topic><topic>Epidermis - physiology</topic><topic>Episkin</topic><topic>Fatty Acids - analysis</topic><topic>Female</topic><topic>Full thickness skin</topic><topic>General pharmacology</topic><topic>Heat-separated human epidermis</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Permeability</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Skin - anatomy & histology</topic><topic>Skin - chemistry</topic><topic>Skin lipids</topic><topic>Skin, Artificial</topic><topic>Split thickness skin</topic><topic>Stratum corneum</topic><topic>Testosterone</topic><topic>Tissue Engineering - methods</topic><topic>Triglycerides - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Netzlaff, Frank</creatorcontrib><creatorcontrib>Kaca, Monika</creatorcontrib><creatorcontrib>Bock, Udo</creatorcontrib><creatorcontrib>Haltner-Ukomadu, Eleonore</creatorcontrib><creatorcontrib>Meiers, Peter</creatorcontrib><creatorcontrib>Lehr, Claus-Michael</creatorcontrib><creatorcontrib>Schaefer, Ulrich F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Netzlaff, Frank</au><au>Kaca, Monika</au><au>Bock, Udo</au><au>Haltner-Ukomadu, Eleonore</au><au>Meiers, Peter</au><au>Lehr, Claus-Michael</au><au>Schaefer, Ulrich F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Permeability of the reconstructed human epidermis model Episkin ® in comparison to various human skin preparations</atitle><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle><addtitle>Eur J Pharm Biopharm</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>66</volume><issue>1</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0939-6411</issn><eissn>1873-3441</eissn><abstract>The objective of this work was to compare the barrier function of the small diameter reconstructed human epidermis model Episkin
® (
d
=
12
mm) to human skin
in vitro. For that purpose a modification for the Franz diffusion cell (
d
=
15
mm) had to be developed so as to allow direct comparison with the following human skin preparations: Full thickness skin (FTS), split thickness skin (STS), heat-separated epidermis (HSE), and trypsin isolated stratum corneum (TISC). Among the tested preparations, HSE appeared to be the most preferable due to its clear morphological structure and ease of preparation. The lipid profile of HSE and Episkin
® was analyzed and showed significant differences in terms of cholesterol, ceramides and triglycerides contents, whereas cholesterol esters and fatty acids were not different. Permeation data with HSE and Episkin
® were then gathered using caffeine and testosterone. Both test compounds permeated much faster through Episkin
® than through HSE. Moreover, opposed to Episkin
®, HSE differentiated between the two test compounds. In spite of the remarkable progress in developing RHEs in the past years at this time Episkin
® can obviously not yet fully replace human skin for
in vitro permeability experiments.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17029766</pmid><doi>10.1016/j.ejpb.2006.08.012</doi><tpages>8</tpages></addata></record> |
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identifier | ISSN: 0939-6411 |
ispartof | European journal of pharmaceutics and biopharmaceutics, 2007-04, Vol.66 (1), p.127-134 |
issn | 0939-6411 1873-3441 |
language | eng |
recordid | cdi_proquest_miscellaneous_70283319 |
source | ScienceDirect Freedom Collection 2022-2024 |
subjects | Biological and medical sciences Caffeine Ceramides - analysis Cholesterol Esters - analysis Chromatography, High Pressure Liquid Collagen - analysis Epidermis - anatomy & histology Epidermis - chemistry Epidermis - physiology Episkin Fatty Acids - analysis Female Full thickness skin General pharmacology Heat-separated human epidermis Hot Temperature Humans Medical sciences Permeability Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Skin - anatomy & histology Skin - chemistry Skin lipids Skin, Artificial Split thickness skin Stratum corneum Testosterone Tissue Engineering - methods Triglycerides - analysis |
title | Permeability of the reconstructed human epidermis model Episkin ® in comparison to various human skin preparations |
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