Significance of TWIST expression and its association with E-cadherin in bladder cancer

• Published in: Human pathology 2007-04, Vol.38 (4), p.598-606
• Main Authors: Zhang, Zheng, MD, Xie, Dan, PhD, Li, Xin, MD, Wong, Yogn-Chuan, PhD, Xin, Dianqi, PhD, Guan, Xin-Yuan, PhD, Chua, Chee Wai, M Phil, Leung, Steve C.L., M Phil, Na, Yanqun, MD, Wang, Xianghong, PhD
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Biomarkers, Tumor - biosynthesis; Bladder; Bladder cancer; Cadherins - biosynthesis; Cancer; Cell adhesion & migration; Chemotherapy; Down-Regulation; E-cadherin; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Lymphatic Metastasis - physiopathology; Male; Medical sciences; Metastasis; Middle Aged; Nephrology. Urinary tract diseases; Nuclear Proteins - biosynthesis; Pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proteins; RNA, Messenger - metabolism; Tumors; Tumors of the urinary system; TWIST; Twist-Related Protein 1 - biosynthesis; Up-Regulation; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Urinary tract. Prostate gland
• ISSN: 0046-8177; 1532-8392
• DOI: 10.1016/j.humpath.2006.10.004

Summary Recently, TWIST, a basic helix-loop-helix transcription factor, has been reported to play a key role in the metastatic progression of several types of human cancer. The aim of this study was to investigate the significance of TWIST expression in bladder cancer using tissue microassays generated from 226 bladder tissue specimens. Using immunohistochemical staining, we studied TWIST expression levels in nonmalignant bladder tissues (n = 37), primary bladder cancer tissues (n = 164), and 25 cases of matched lymph node metastatic lesions. The association between TWIST expression levels and tumor staging and grading, as well as metastatic potential, was analyzed by statistical analysis. Our results showed that TWIST protein expression was significantly higher in bladder cancer specimens compared with nonmalignant tissues ( P < .001), indicating its positive role in the development of bladder cancer. In addition, increased TWIST expression levels were associated with advanced-stage and high-grade tumors, suggesting its involvement in the progression of this cancer. Furthermore, TWIST expression was much higher in the metastatic lesion compared with its primary site ( P < .05). More importantly, the increased TWIST expression in bladder cancer specimens was correlated with decreased membranous expression of E-cadherin, a cell adhesion molecule that plays a key role in the metastatic progression of human cancer. Our results demonstrate TWIST as a novel positive factor in the development and progression of bladder cancer and suggest a marker for advanced bladder cancer.