Expression of nebulette during early cardiac development

Nebulette, a cardiac homologue of nebulin, colocalizes with α‐actinin in the pre‐myofibrils of spreading cardiomyocytes and has been hypothesized to play a critical role in the formation of the thin‐filament‐Z‐line complex early during myofibrillogenesis. Data from mesodermal explants or whole tissu...

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Bibliographic Details
Published in:Cell motility and the cytoskeleton 2007-04, Vol.64 (4), p.258-273
Main Authors: Esham, Michael, Bryan, Kourtney, Milnes, Jennifer, Holmes, William B., Moncman, Carole L.
Format: Article
Language:English
Subjects:
Actinin - biosynthesis
Actinin - ultrastructure
Animals
Carrier Proteins - biosynthesis
Chick Embryo
Cytoskeletal Proteins - biosynthesis
Heart - embryology
Heart - growth & development
Humans
LIM Domain Proteins
Muscle Development - physiology
Myocardium - metabolism
Myocytes, Cardiac - cytology
Myocytes, Cardiac - metabolism
nebulin
thin filaments
Z-line
α-actinin
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Summary:Nebulette, a cardiac homologue of nebulin, colocalizes with α‐actinin in the pre‐myofibrils of spreading cardiomyocytes and has been hypothesized to play a critical role in the formation of the thin‐filament‐Z‐line complex early during myofibrillogenesis. Data from mesodermal explants or whole tissue mounts of developing hearts suggest that the pattern of myofibrillogenesis in situ may differ from observations of spreading cardiomyocytes. To evaluate the role of nebulette in myofibrillogenesis, we have analyzed the expression of nebulette in chicken heart rudiments by immunoblots and immunofluorescence. We detect the 110 kDa nebulette in heart rudiments derived from stage 9–10 using the anti‐nebulin mAb, N114, or polyclonal anti‐nebulette Abs by immunoblotting. Immunofluorescence analysis of explants stained with anti‐nebulette and anti‐α‐actinin Abs demonstrates that both proteins localize along actin filaments in punctate to continuous manner at early stages of cardiac development and later give rise to striations. In both cases, the punctate staining had a periodicity of ∼1.0 μm indicating a pre‐myofibrils distribution at the earliest time points examined. We demonstrate that nebulette is indeed associated with premyofibrils in very early stages of myofibrillogenesis and suggest that nebulette may play an important role in the formation of these structures. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.
ISSN:0886-1544
1097-0169
DOI:10.1002/cm.20180