Phenotypic expression of human hepatoma cells in culture

Hepatomas thrive in a hypoxic environment resulting in the induction of a cluster of hypoxia related genes. The protein phenotypic expression include hypoxia inducible factor‐α, prolyl‐4‐hydroxylase, vascular endothelear growth factor and erythropoietin. The present study was undertaken to determine...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2007-04, Vol.100 (5), p.1081-1085
Main Authors: Cutroneo, Kenneth R., White, Sheryl L., Buttolph, Thomas R., Allison, Gretchen, Ehrlich, H. Paul
Format: Article
Language:English
Subjects:
Carcinoma, Hepatocellular - metabolism
Cell Hypoxia
erythropoetin
Erythropoietin - metabolism
human hepatoma cells (Hep 2G)
Humans
Hypoxia-Inducible Factor 1, alpha Subunit - metabolism
hypoxia-inducible factor-1α
Liver Neoplasms - metabolism
Phenotype
Procollagen-Proline Dioxygenase - metabolism
Tumor Cells, Cultured
vascular endothelial growth factor
Vascular Endothelial Growth Factor A - metabolism
β-subunit of prolyl-4-hydorxylase
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Hepatomas thrive in a hypoxic environment resulting in the induction of a cluster of hypoxia related genes. The protein phenotypic expression include hypoxia inducible factor‐α, prolyl‐4‐hydroxylase, vascular endothelear growth factor and erythropoietin. The present study was undertaken to determine if human hepatoma cells when cultured for 72 h in the presence of serum under normoxia would maintain their cancerous phenotypic expression of certain hypoxia inducible genes. Our positive results affords an in vitro model system to test hypoxia inhibitors on the expression and the intracellular compartmentalization or the secretion of these hypoxia‐inducible proteins. J. Cell. Biochem. 100: 1081–1085, 2007. © 2007 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.21150