Identification of mesenchymal stem cells in aorta-gonad-mesonephros and yolk sac of human embryos

Mesenchymal stem cells (MSCs) are multipotent stem cells that can generate various microenvironment components in bone marrow, ensuring a precise control over self-renewal and multilineage differentiation of hematopoietic stem cells. Nevertheless, their spatiotemporal correlation with embryonic hema...

Full description

Saved in:
Bibliographic Details
Published in:Blood 2008-02, Vol.111 (4), p.2436-2443
Main Authors: Wang, Xiao-Yan, Lan, Yu, He, Wen-Yan, Zhang, Lei, Yao, Hui-Yu, Hou, Chun-Mei, Tong, Ying, Liu, Yuan-Lin, Yang, Guan, Liu, Xiao-Dan, Yang, Xiao, Liu, Bing, Mao, Ning
Format: Article
Language:English
Subjects:
Abortion, Induced
Antigens, CD - analysis
Aorta - cytology
Aorta - embryology
Biological and medical sciences
Cell Differentiation - physiology
Clone Cells
Dissection
Embryonic Development
Female
Flow Cytometry
Hematologic and hematopoietic diseases
Humans
Medical sciences
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - physiology
Mesonephros - cytology
Pregnancy
Yolk Sac - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mesenchymal stem cells (MSCs) are multipotent stem cells that can generate various microenvironment components in bone marrow, ensuring a precise control over self-renewal and multilineage differentiation of hematopoietic stem cells. Nevertheless, their spatiotemporal correlation with embryonic hematopoiesis remains rudimentary, particularly in relation to the human being. Here, we reported that human aorta-gonad-mesonephros (AGM) resided with bona fide MSCs. They were highly proliferative as fibroblastoid population bearing uniform surface markers (CD45−, CD34−, CD105+, CD73+, CD29+, and CD44+), expressed pluripotential molecules Oct-4 and Nanog, and clonally demonstrated trilineage differentiation capacity (osteocytes, chondrocytes, and adipocytes). The frequency and absolute number of MSCs in aorta plus surrounding mesenchyme (E26-E27) were 0.3% and 164, respectively. Moreover, they were functionally equivalent to MSCs from adult bone marrow, that is, supporting long-term hematopoiesis and suppressing T-lymphocyte proliferation in vitro. In comparison, the matching yolk sac contained bipotent mesenchymal precursors that propagated more slowly and failed to generate chondrocytes in vitro. Together with previous knowledge, we propose that a proportion of MSCs initially develop in human AGM prior to their emergence in embryonic circulation and fetal liver.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2007-07-099333