Experimental infection of macaques with human metapneumovirus induces transient protective immunity

Department of Virology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands Correspondence Bernadette G. van den Hoogen b.vandenhoogen{at}erasmusmc.nl Human metapneumovirus (hMPV), a member of the family Paramyxoviridae , is a causative agent of acute respiratory-tract illness. Two main hMPV...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 2007-04, Vol.88 (4), p.1251-1259
Main Authors: van den Hoogen, Bernadette G, Herfst, Sander, de Graaf, Miranda, Sprong, Leo, van Lavieren, Rob, van Amerongen, Geert, Yuksel, Selma, Fouchier, Ron A. M, Osterhaus, Albert D. M. E, de Swart, Rik L
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
Biological and medical sciences
Cynomolgus
Fundamental and applied biological sciences. Psychology
Human metapneumovirus
Macaca
Macaca fascicularis
Metapneumovirus - immunology
Microbiology
Miscellaneous
Neutralization Tests
Nose - virology
Paramyxoviridae
Paramyxoviridae Infections - immunology
Paramyxovirus
Pharynx - virology
Virology
Virus Shedding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Department of Virology, Erasmus MC, PO Box 2040, 3000 CA Rotterdam, The Netherlands Correspondence Bernadette G. van den Hoogen b.vandenhoogen{at}erasmusmc.nl Human metapneumovirus (hMPV), a member of the family Paramyxoviridae , is a causative agent of acute respiratory-tract illness. Two main hMPV lineages circulate worldwide and reinfections occur frequently. It is unclear what level of protection is induced by natural hMPV infection, what the durability of this protection is and whether it differs for reinfection with homologous or heterologous viruses. Here, protective immunity in cynomolgus macaques at different time points after inoculation with molecularly cloned prototype viruses of the two main lineages of hMPV has been addressed. Animals received a homologous challenge at 4, 6 or 12 weeks after the primary infection. In addition, animals that had been inoculated three times within 10 weeks were challenged with homologous or heterologous virus 8 months later. Primary infection with 10 7 TCID 50 resulted in virus shedding and induction of virus-neutralizing antibody responses, with higher titres against the homologous than the heterologous virus. Infections associated with virus shedding and seroconversion protected completely from homologous reinfection within 6 weeks, and partly at 12 weeks, after primary infection. Eight months later, protection had waned to virtually undetectable levels. This study demonstrates that experimental hMPV infection induces transient protective immunity.
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.82663-0