Effects of insufficient air injection on methanogenic Archaea in landfill bioreactor

In this study, methanogenic Archaea diversity in an aerated landfill bioreactor filled with co-disposed incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time using molecular techniques. Besides, the effects of insufficient air injection on the b...

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Bibliographic Details
Published in:Journal of hazardous materials 2007-04, Vol.142 (1), p.258-265
Main Authors: Mertoglu, Bulent, Calli, Baris, Guler, Nuray, Inanc, Bulent, Inoue, Yuzo
Format: Article
Language:English
Subjects:
Aeration
Air
Applied sciences
Archaea
Archaea - classification
Archaea - genetics
Archaea - physiology
Base Sequence
Biological and medical sciences
Bioreactors
Biotechnology
Chemical engineering
Colony Count, Microbial
DNA Primers
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General treatment and storage processes
Incineration ash
Landfill
Methane - biosynthesis
Methanogen
Methanomicrobiales
Methods. Procedures. Technologies
Molecular methods
Nucleic Acid Hybridization
Others
Phylogeny
Pollution
Polymerase Chain Reaction
Reactors
Refuse Disposal
RNA, Ribosomal, 16S - genetics
Various methods and equipments
Wastes
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Summary:In this study, methanogenic Archaea diversity in an aerated landfill bioreactor filled with co-disposed incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time using molecular techniques. Besides, the effects of insufficient air injection on the bioreactor performance and methanogenic diversity were evaluated thoroughly. Results indicated that rapid bio-stabilization of solid waste are possible with aerated landfill bioreactor at various oxygen and oxidation reduction potential levels. Slot-blot hybridization results of leachate samples collected from aerated landfill bioreactor showed that archaeal and bacterial activities increased as stabilization accelerated and bacterial populations constituted almost 95% of all microorganisms. The results of slot-blot hybridization and phylogenetic analysis based on 16S rRNA gene revealed that Methanobacteriales and Methanomicrobiales were dominant species at the beginning while substituted by Methanosarcina-related methanogens close to the end of the operation of bioreactor.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2006.08.008