A DNA−Gold Nanoparticle-Based Colorimetric Competition Assay for the Detection of Cysteine

We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine−thymidine (T−T) mismatches complexed with Hg2+. This assay relies upon the distance-...

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Bibliographic Details
Published in:Nano letters 2008-02, Vol.8 (2), p.529-533
Main Authors: Lee, Jae-Seung, Ulmann, Pirmin A, Han, Min Su, Mirkin, Chad A
Format: Article
Language:English
Subjects:
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Colorimetry - instrumentation
Colorimetry - methods
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Cross-disciplinary physics: materials science
rheology
Crystallization - methods
Cysteine - analysis
Cysteine - chemistry
DNA - chemistry
Equipment Design
Equipment Failure Analysis
Exact sciences and technology
Gold - chemistry
Macromolecular Substances - chemistry
Materials science
Materials Testing
Molecular Conformation
Nanocrystalline materials
Nanoscale materials and structures: fabrication and characterization
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation
Optical properties of low-dimensional, mesoscopic, and nanoscale materials and structures
Particle Size
Physics
Surface Properties
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Summary:We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine−thymidine (T−T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV−vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl0727563