Expression of the MAST family of serine/threonine kinases

Abstract The Microtubule-Associated Serine/Threonine Kinase family (MAST1–4, and MAST-like) is characterised by the presence of a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain (PDZ). This latter domain gives the MAST family the capacity to s...

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Bibliographic Details
Published in:Brain research 2008-02, Vol.1195, p.12-19
Main Authors: Garland, Patrick, Quraishe, Shmma, French, Pim, O'Connor, Vincent
Format: Article
Language:English
Subjects:
Animals
Biochemistry and metabolism
Biological and medical sciences
Brain - enzymology
Central nervous system
Dystrophin
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
In situ hybridisation
Male
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Myocardium - enzymology
Neurology
Oligodendrocytes
PDZ domain
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Rats
Rats, Wistar
RNA, Messenger - analysis
Seizure
Signal Transduction - physiology
Striatum
Testis - enzymology
Tissue Distribution
Vertebrates: nervous system and sense organs
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Summary:Abstract The Microtubule-Associated Serine/Threonine Kinase family (MAST1–4, and MAST-like) is characterised by the presence of a serine/threonine kinase domain and a postsynaptic density protein-95/discs large/zona occludens-1 domain (PDZ). This latter domain gives the MAST family the capacity to scaffold its own kinase activity. In the present study we have profiled the mRNA for each member of the MAST family transcripts across various tissues, with particular focus on rodent brain. Reverse-transcriptase polymerase chain reaction (RT-PCR) has shown equivalent patterns of expression for MAST1 and 2 in multiple tissues. Both MAST3 and 4 show more distinct expression in several tissues, and MAST-like appears to be predominantly expressed in heart and testis. In situ hybridisation reveals overlapping expression of MAST1 and 2 in specific brain regions. In contrast, MAST3 shows selective expression in the striatum and cerebral cortex. MAST4 also exhibits distinct expression in oligodendrocytes of white matter containing brain regions. In keeping with previous results, this family member also shows increased expression in the hippocampus following seizure-like activity. Our analysis of MAST family expression provides support for the role of these kinases in a broad range of neural functions.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2007.12.027