Genotypic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Isolates Recovered From Central China

DNA sequencing analysis was used to investigate genetic alterations in the rpoB, katG, and inhA regulatory region and embB in 66 Mycobacterium tuberculosis isolates recovered from Central China. Of the 36 multidrug-resistant isolates, 33 (92%) had mutations in the amplified region of rpoB. The most...

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Bibliographic Details
Published in:Biochemical genetics 2007-04, Vol.45 (3-4), p.281-290
Main Authors: Zhang, Shu-Lin, Qi, Hua, Qiu, Dun-Lian, Li, Da-Xu, Zhang, Jie, Du, Chang-Mei, Wang, Guo-Bin, Yang, Zhi-Rong, Sun, Qun
Format: Article
Language:English
Subjects:
China
Codons
DNA sequencing
Drug resistance
Drug Resistance, Multiple, Bacterial - genetics
genes
Genotype
Geographical variations
Humans
multidrug resistant
Mutation
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Mycobacterium tuberculosis - isolation & purification
Phenotype
Regulatory sequences
RpoB protein
sequence
Tuberculosis
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Summary:DNA sequencing analysis was used to investigate genetic alterations in the rpoB, katG, and inhA regulatory region and embB in 66 Mycobacterium tuberculosis isolates recovered from Central China. Of the 36 multidrug-resistant isolates, 33 (92%) had mutations in the amplified region of rpoB. The most frequent mutation (58%, 19/36) was S531L (TCG[rightward arrow]TTG). At least one mutation was found in the katG and inhA regulatory region in 83% (30/36) of the multidrug-resistant isolates, and mutations at katG codon 315 were identified in 78% (28/36). Alterations at embB306 may not confer resistance to EMB, and embB306 mutants were more frequently accompanied by rpoB mutations (100%, 16/16) than by katG 315 mutations (75%, 12/16). Our results show that geographic variation in the molecular genetic mechanism is responsible for drug resistance in multidrug-resistant M. tuberculosis. This observation will facilitate the development of a rapid molecular drug resistance screening approach for drug-resistant M. tuberculosis.
ISSN:0006-2928
1573-4927
DOI:10.1007/s10528-006-9074-6