Preparation of biomolecule gel matrices for electron microscopy

We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural ch...

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Bibliographic Details
Published in:Ultramicroscopy 2008-03, Vol.108 (4), p.309-313
Main Authors: Padalkar, Sonal, Zhao, Jianxiu, Stuart, Kate, Panitch, Alyssa, Rickus, Jenna, Stanciu, Lia
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Biomolecules
Collagen Type I - ultrastructure
Collagen Type III - ultrastructure
Electron microscopy
Gels
Liposomes - chemistry
Microscopy, Electron, Transmission - methods
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Summary:We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule–gel matrix systems.
ISSN:0304-3991
1879-2723
DOI:10.1016/j.ultramic.2007.04.004