Specificity Analysis of Anti-gliadin Mouse Monoclonal Antibodies Used for Detection of Gliadin in Food for Gluten-free Diet

A gluten-free diet (GFD) is the sole effective long-lasting treatment of celiac disease. Four monoclonal antibodies (Abs) were prepared by immunization of animals kept on GFD with gliadin. The specificity of these Abs to decapeptides of α- and γ-gliadin and ω-secalin was analyzed by the PEPSCAN tech...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-04, Vol.55 (7), p.2627-2632
Main Authors: Sánchez, Daniel, Tučková, Ludmila, Burkhard, Martin, Plicka, Jan, Mothes, Thomas, Hoffmanová, Iva, Tlaskalová-Hogenová, Helena
Format: Article
Language:English
Subjects:
Amino Acid Sequence
analytical kits
analytical methods
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Biological and medical sciences
Celiac Disease - diet therapy
Cereal and baking product industries
decapeptides
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
food analysis
Food Analysis - methods
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
gliadin
Gliadin - analysis
Gliadin - chemistry
Gliadin - immunology
gluten
gluten-free foods
Glutens - analysis
Methods of analysis, processing and quality control, regulation, standards
Mice
monoclonal antibodies
peptides
secalins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A gluten-free diet (GFD) is the sole effective long-lasting treatment of celiac disease. Four monoclonal antibodies (Abs) were prepared by immunization of animals kept on GFD with gliadin. The specificity of these Abs to decapeptides of α- and γ-gliadin and ω-secalin was analyzed by the PEPSCAN technique. Repetitive sequences of α- and γ-gliadin and ω-secalin containing the motifs QPFPXQ (X = Q, L, P) were recognized by all Abs tested. These Abs also frequently reacted with peptides containing the sequences QQSFPQQ, QQTFPQP, and QPFRPQ. On the basis of PEPSCAN results two Abs8D4 and 7C6were selected for the construction of a new ELISA kit for the detection of gliadin in food. The comparison of data obtained using the newly developed ELISA kit and commercially available ones indicated that Abs selection on the basis of their fine specificity to gliadin is useful for sensitive detection of gliadin in foods. Keywords: Antibody specificity; gliadin; celiac disease
ISSN:0021-8561
1520-5118
DOI:10.1021/jf0630421