Methods for extracting biochemical information from bacterial Raman spectra: An explorative study on Cupriavidus metallidurans

In this study, we explore Raman spectra of bacteria for their biochemical information. Therefore, a database of biomolecules was used and several approaches were applied such as the study of difference spectra, the calculation dot products, the usage of coefficients obtained from an EMSC procedure a...

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Bibliographic Details
Published in:Analytica chimica acta 2007-03, Vol.585 (2), p.234-240
Main Authors: De Gelder, Joke, De Gussem, Kris, Vandenabeele, Peter, De Vos, Paul, Moens, Luc
Format: Article
Language:English
Subjects:
2D correlation spectroscopy
Acetyl Coenzyme A - chemistry
Analytical chemistry
Bacteria
Bacteria - metabolism
Chemistry
Chemistry Techniques, Analytical - methods
Citric Acid - chemistry
Citric Acid Cycle
Cupriavidus - metabolism
Cupriavidus metallidurans
EMSC
Exact sciences and technology
Metabolism
Models, Chemical
Phenylalanine - chemistry
Raman spectroscopy
Spectrometric and optical methods
Spectrum Analysis, Raman - methods
Tyrosine - chemistry
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Summary:In this study, we explore Raman spectra of bacteria for their biochemical information. Therefore, a database of biomolecules was used and several approaches were applied such as the study of difference spectra, the calculation dot products, the usage of coefficients obtained from an EMSC procedure and the application of 2D correlation spectroscopy. These methods were applied on a dataset containing Raman spectra of Cupriavidus metallidurans LMG 1195 in five stages of its growth, aiming to extract information about the evolution of cell components during growth. EMSC coefficients seemed to be most promising for tracking metabolic products and the results were often confirmed by difference spectra or by 2D correlation spectroscopy.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2006.12.050