16S rRNA Gene Sequences from Bacteria Associated with Adult Anopheles darlingi (Diptera: Culicidae) Mosquitoes

• Published in: Journal of medical entomology 2008-01, Vol.45 (1), p.172-175
• Main Authors: Terenius, Olle, Dantas de Oliveira, Caroline, Pinheiro, Waleria Dasso, Tadei, Wanderli Pedro, James, Anthony Amade, Marinotti, Osvaldo
• Format: Article
• Language: English
• Subjects: Animals; Anopheles - microbiology; Anopheles darlingi; bacteria; Bacteria - classification; Bacteria - genetics; Biological and medical sciences; disease vectors; Enterobacteriaceae; Female; Fundamental and applied biological sciences. Psychology; Gastrointestinal Tract - microbiology; Insecta; Invertebrates; malaria; Male; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; microorganisms; paratransgenesis; Phylogeny; polymerase chain reaction; ribosomal DNA; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; sequence analysis; sequence homology; Short Communications; taxonomy; Vectors. Intermediate hosts
• ISSN: 0022-2585; 1938-2928
• DOI: 10.1603/0022-2585%282008%2945%5B172%3ASRGSFB%5D2.0.CO%3B2

The microbial flora associated with Anopheles darlingi Root (Diptera: Culicidae), a major Neotropical malaria vector, was investigated for the development of a paratransgenesis-based approach to control malaria transmission in Brazil. Female mosquitoes were collected using human land catches and captured insects provided a bloodmeal. The controlled blood feeding resulted in increased detection of mosquito bacterial population because it was possible to retrieve bacterial DNA from all blood-fed mosquitoes. The 16S sequences of bacteria recovered, include some closely related to those found in other vector mosquitoes, including Aeromonas, Pantoea and Pseudomonas species.