A novel detection approach based on chromophore-decolorizing with free radical and application to photometric determination of copper with acid chrome dark blue

• Published in: Analytica chimica acta 2007-03, Vol.587 (1), p.52-59
• Main Authors: Gao, Hong-Wen, Chen, Fang-Fang, Chen, Ling, Zeng, Teng, Pan, Lu-Ting, Li, Jian-Hua, Luo, Hua-Fei
• Format: Article
• Language: English
• Subjects: Acid chrome dark blue; Analytical chemistry; Azo Compounds - chemistry; Chemistry; Chromophore-decolorizing; Copper - chemistry; Determination of copper; DNA - chemistry; Electrochemistry - methods; Exact sciences and technology; Free Radicals - chemistry; Hydrogen Peroxide - chemistry; Hydrogen-Ion Concentration; Hydroxyl radicals; Indicators and Reagents - chemistry; Indicators and Reagents - pharmacology; Iron - chemistry; Models, Chemical; Naphthalenesulfonates - chemistry; Photometry - methods; Sensitivity and Specificity; Spectral correction technique; Spectrometric and optical methods; Spectrophotometry; Spectrophotometry - methods; Trace Elements - chemistry
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2007.01.046

A novel detection approach named chromophore-decolorizing with free radicals is developed for determination of trace heavy metal. The hydroxyl radicals (HO ) generated from Fe(III) and hydrogen peroxide will oxidize the free chromophore into almost colorless products. The copper–acid chrome dark blue (ACDB) complexation was investigated at pH 5.07. In the presence of Fe(III) and hydrogen peroxide, the excess ACDB was decolorized in the Cu–ACDB reaction solution, and the final solution contained only one color compound, the Cu–ACDB complex. After oxidation of free hydroxyl radicals, the complexation becomes sensitive and selective and it has been used for the quantitation of trace amounts of Cu(II) dissolved in natural water. Beer's law is obeyed in the range from 0 to 0.500 μg mL −1 Cu(II) and the limit of detection is only 6 μg L −1 Cu(II). Besides, the Cu–ACDB complex formed was characterized.