Analytical Performances of Aptamer-Based Sensing for Thrombin Detection

Aptamer-based assays represent a modern and attractive approach in bioanalytical chemistry. The DNA thrombin aptamer has been extensively investigated, and the coupling of this aptamer to different transduction principles has demonstrated the wide applicability of aptamers as bioreceptors in bioanal...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2007-04, Vol.79 (7), p.3016-3019
Main Authors: Bini, Alessandra, Minunni, Maria, Tombelli, Sara, Centi, Sonia, Mascini, Marco
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Aptamers, Nucleotide - chemistry
Biological and medical sciences
Biosensors
Chemistry
Deoxyribonucleic acid
DNA
Electrodes
Enzymes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Gold - chemistry
Humans
Ribozymes
Serum Albumin - chemistry
Signal transduction
Surface Properties
Thrombin - analysis
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aptamer-based assays represent a modern and attractive approach in bioanalytical chemistry. The DNA thrombin aptamer has been extensively investigated, and the coupling of this aptamer to different transduction principles has demonstrated the wide applicability of aptamers as bioreceptors in bioanalytical assays. The goal of this work was to critically evaluate all the parameters that can influence the sensor performances by using the thrombin aptamer immobilized onto piezoelectric quartz crystals. The optimization of the immobilization and the binding protocol was of paramount importance, and improvements in analytical performances could be obtained by optimizing simple steps in immobilization and assay conditions. Moreover, the work demonstrated the possibility of using aptamer-based sensors in complex matrixes, opening the possibility of a real application to diagnostics or medical investigation.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac070096g