Resistance of human leukocytes to vesicular stomatitis virus infection as one of the innate antiviral immune activities; participation of cell subpopulations

Among reactions of innate immunity, resistance of human peripheral blood leukocytes (PBL) to viral infection seems important. The purpose of our study was to find, which of the subpopulations of PBL is the most responsible for the innate antiviral immunity of these cells. The innate immunity was mea...

Full description

Saved in:
Bibliographic Details
Published in:Folia histochemica et cytobiologica 2008-01, Vol.46 (1), p.39-43
Main Authors: Zaczynska, Ewa, Duś, Danuta, Paprocka, Maria, Błach-Olszewska, Zofia
Format: Article
Language:English
Subjects:
Adolescent
Adult
Cell Separation
Flow Cytometry
Humans
Immunity, Innate - immunology
Killer Cells, Natural - cytology
Killer Cells, Natural - immunology
Kinetics
Leukocytes - cytology
Leukocytes - immunology
Leukocytes - virology
Middle Aged
Rhabdoviridae Infections - immunology
Vesicular stomatitis Indiana virus - immunology
Vesicular stomatitis Indiana virus - physiology
Virus Replication
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among reactions of innate immunity, resistance of human peripheral blood leukocytes (PBL) to viral infection seems important. The purpose of our study was to find, which of the subpopulations of PBL is the most responsible for the innate antiviral immunity of these cells. The innate immunity was measured by using the direct method of infection of leukocytes with vesicular stomatitis virus (VSV). The lack of VSV replication by infected leukocytes (0-1 log TCID50) was taken as an indicator for complete immunity; a low level of VSV (2-3 log) for partial immunity; and high VSV titer (more than 4 log) for no immunity. The resistance/innate immunity of whole PBL and subpopulations such as: adherent cells, fractions enriched in lymphocytes T, and lymphocytes B (separated on column with nylon wool), NK(+) and NK(-) (separated by microbeads activated cell sorting MACS) differ from each other. All fractions express higher resistance/innate immunity than the whole PBL. NK(+) cells were found the most resistant fraction of PBL to VSV infection. The results indicate that among the leukocytes in PBL the regulation mechanisms of innate immunity exist. The study on the mechanism of innate immunity regulation as well as the role of NK in innate immunity of PBL must be continued.
ISSN:0239-8508
1897-5631
DOI:10.2478/v10042-008-0004-9