One-dimensional microfluidic beads array for multiple mRNAs expression detection

• Published in: Biosensors & bioelectronics 2007-05, Vol.22 (11), p.2759-2762
• Main Authors: Wen, Jianhui, Yang, Xiaohai, Wang, Kemin, Tan, Weihong, Zhou, Leiji, Zuo, Xinbing, Zhang, He, Chen, Yunqing
• Format: Article
• Language: English
• Subjects: Beads array; Biological and medical sciences; Biotechnology; Cancer cell; Cells, Cultured; Epithelial Cells - metabolism; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - instrumentation; Gene Expression Profiling - methods; Humans; In Situ Hybridization, Fluorescence - instrumentation; In Situ Hybridization, Fluorescence - methods; Microfluidic; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Array Sequence Analysis - methods; RNA, Messenger - analysis; RNA, Messenger - genetics; Transcriptional expression
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2006.11.029

A one-dimensional microfluidic beads array for in vitro rapid measurement of multiple mRNAs expression is presented in this paper. Gene specific capture DNA-functional beads were deposited along a microchannel to form an addressable beads array. We demonstrated that the one-dimensional beads array could perform simultaneous multiple nucleic acid targets detection and a DNA detection limit of 0.02 nM was obtained. Using this array, transcripts expression of three tumor-associated genes, including p53, H-ras, and NME1, both in CNE2 nasopharyngeal carcinoma cell lines and in normal human nasopharyngeal epithelial cells were evaluated. The responses of these three genes expression in CNE2 cells to 5-flouorouracil (5-Fu) stimuli were also assessed. Validation of these results was performed using reverse transcriptase-PCR. The presented methodology combines high throughput of microarrays and low sample consumption, convenient liquid handling of microfluidics. It enables rapid and facile determination of multi-gene expression and holds great potential in early cancer diagnostics and molecular biology.