Early detection of bean infection by Pseudomonas syringae in asymptomatic leaf areas using chlorophyll fluorescence imaging

Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom...

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Bibliographic Details
Published in:Photosynthesis research 2008-04, Vol.96 (1), p.27-35
Main Authors: Rodríguez-Moreno, Luis, Pineda, Mónica, Soukupová, Julia, Macho, Alberto P, Beuzón, Carmen R, Barón, Matilde, Ramos, Cayo
Format: Article
Language:English
Subjects:
Beans
Biochemistry
Biomedical and Life Sciences
Chlorophyll
Chlorophyll - chemistry
Chlorophyll - metabolism
Fluorescence
Life Sciences
Lycopersicon esculentum
Phaseolus - metabolism
Phaseolus - microbiology
Phaseolus - physiology
Phaseolus vulgaris
Photosynthesis
Photosynthesis - physiology
Plant diseases
Plant Genetics and Genomics
Plant Leaves - metabolism
Plant Leaves - microbiology
Plant Physiology
Plant Sciences
Pseudomonas syringae
Pseudomonas syringae - growth & development
Regular Paper
Scientific imaging
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Summary:Chlorophyll fluorescence imaging has been used to analyse the response elicited in Phaseolus vulgaris after inoculation with Pseudomonas syringae pv. phaseolicola 1448A (compatible interaction) and P. syringae pv. tomato DC3000 (incompatible interaction). With the aim of modulating timing of symptom development, different cell densities were used to inoculate bean plants and the population dynamics of both bacterial strains was followed within the leaf tissue. Fluorescence quenching analysis was carried out and images of the different chlorophyll fluorescence parameters were obtained for infected as well as control plants at different timepoints post-infection. Among the different parameters analysed, we observed that non-photochemical quenching maximised the differences between the compatible and the incompatible interaction before the appearance of visual symptom. A decrease in non-photochemical quenching, evident in both infiltrated and non-infiltrated leaf areas, was observed in P. syringae pv. phaseolicola-infected plants as compared with corresponding values from controls and P. syringae pv. tomato-infected plants. No photoinhibitory damage was detected, as the maximum photosystem II quantum yield remained stable during the infection period analysed.
ISSN:0166-8595
1573-5079
DOI:10.1007/s11120-007-9278-6