Suppressive role of leukocyte cell–derived chemotaxin 2 in mouse anti–type II collagen antibody–induced arthritis

• Published in: Arthritis and rheumatism 2008-02, Vol.58 (2), p.413-421
• Main Authors: Okumura, Akinori, Saito, Takeshi, Otani, Isao, Kojima, Kazuo, Yamada, Yasunori, Ishida‐Okawara, Akiko, Nakazato, Katsuyoshi, Asano, Masahide, Kanayama, Kiichi, Iwakura, Yoichiro, Suzuki, Kazuo, Yamagoe, Satoshi
• Format: Article
• Language: English
• Subjects: Animals; Arthritis, Experimental - genetics; Arthritis, Experimental - immunology; Arthritis, Experimental - pathology; Autoantibodies - pharmacology; Biological and medical sciences; Chemokine CCL3 - immunology; Diseases of the osteoarticular system; Gene Expression - immunology; Inflammatory joint diseases; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - immunology; Interleukin-1beta - immunology; Lipopolysaccharides - pharmacology; Male; Medical sciences; Mice; Mice, Inbred BALB C; Mice, Mutant Strains; Polymorphism, Genetic; Tarsal Joints - pathology; Tumor Necrosis Factor-alpha - immunology
• ISSN: 0004-3591; 1529-0131
• DOI: 10.1002/art.23215

Objective We previously reported that the Val58Ile polymorphism of the leukocyte cell–derived chemotaxin 2 gene (LECT2) is associated with the severity of rheumatoid arthritis (RA). To define the role of LECT2 in inflammatory arthritides, we investigated the development of collagen antibody–induced arthritis (CAIA) in LECT2‐deficient (LECT2−/−) mice. Methods CAIA was induced in mice by administering anti–type II collagen antibodies followed by lipopolysaccharide. Daily assessment of hind paw swelling was used to monitor the development of arthritis. The histopathologic features and expression of inflammatory cytokines were also analyzed. We confirmed the role of LECT2 by introducing a LECT2 expression vector into LECT2−/− mice, using a hydrodynamic gene transfer method. Results Arthritis in LECT2−/− mice was significantly exacerbated compared with that in wild‐type (WT) controls. Histopathologic assessment of the tarsal joints showed that inflammation and erosion of cartilage and bone in LECT2−/− mice were more severe than that in controls. Interleukin‐1β (IL‐1β), IL‐6, and certain chemokines were present at significantly higher levels in the arthritic hind paws of LECT2−/− mice. In contrast, the amount of LECT2 in the serum and locally in the hind paws was higher in arthritic WT mice. Finally, hydrodynamic gene transfer experiments revealed that the severity of arthritis was reduced by the systemic expression of exogenous mouse LECT2 protein in LECT2−/− mice. Conclusion These results strongly suggest that LECT2 directly suppresses the development of CAIA. Manipulation of LECT2 might provide a rationale for novel therapeutic approaches to the treatment of inflammatory arthritides such as RA.