Simple and rapid determination of serum carbapenem concentrations by high-performance liquid chromatography

Abstract Although several high-performance liquid chromatography (HPLC) methods for the determination of serum concentrations of carbapenems have been reported, they are complicated and involve column switching. We established a simple and rapid HPLC method for the determination of serum concentrati...

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Bibliographic Details
Published in:Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2008, Vol.14 (1), p.30-34
Main Authors: Kurihara, Yuji, Kizu, Junko, Hori, Seiji
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - blood
Carbapenems
Carbapenems - administration & dosage
Carbapenems - blood
Carbapenems - pharmacokinetics
Chromatography, High Pressure Liquid - economics
Chromatography, High Pressure Liquid - methods
Hematology, Oncology and Palliative Medicine
High-performance liquid chromatography
Imipenem - blood
Infectious Diseases
Male
Medical Microbiology
Medicine
Medicine & Public Health
Mice
Original Article
Pharmacokinetics
Thienamycins - blood
Virology
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Summary:Abstract Although several high-performance liquid chromatography (HPLC) methods for the determination of serum concentrations of carbapenems have been reported, they are complicated and involve column switching. We established a simple and rapid HPLC method for the determination of serum concentrations of carbapenems, and this method is suitable for routine use in the clinical field. With our HPLC method, the serum concentrations of five commercially available carbapenems could be determined by changing only the methanol/phosphate-buffer ratio in the mobile phase. Serum levels of imipenem, panipenem, and meropenem in mice could be monitored when these carbapenems (20 mg/kg) were administered subcutaneously with cilastatin (20 mg/kg). These results suggest that our simple and rapid HPLC method for the determination of the serum concentrations of carbapenems is useful for pharmacokinetic/pharmacodynamic (PK/PD)-based determination of carbapenem dosage regimens.
ISSN:1341-321X
1437-7780
DOI:10.1007/s10156-007-0576-X