A plant-produced plague vaccine candidate confers protection to monkeys

Abstract Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or huma...

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Bibliographic Details
Published in:Vaccine 2007-04, Vol.25 (16), p.3014-3017
Main Authors: Mett, Vadim, Lyons, Jarred, Musiychuk, Konstantin, Chichester, Jessica A, Brasil, Trevor, Couch, Ronald, Sherwood, Robert, Palmer, Gene A, Streatfield, Stephen J, Yusibov, Vidadi
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antigens
Antigens, Bacterial - biosynthesis
Antigens, Bacterial - metabolism
Applied microbiology
Bacterial diseases
Bacterial Proteins - biosynthesis
Bacteriology
Biological and medical sciences
Clostridium thermocellum
Cynomolgus
Disease Models, Animal
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Genetic Vectors - metabolism
Genomics
Human bacterial diseases
Infectious diseases
Macaca
Macaca fascicularis
Medical sciences
Microbiology
Miscellaneous
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana benthamiana
Pathogens
Plague
Plague - prevention & control
Plague vaccine
Plague Vaccine - genetics
Plague Vaccine - immunology
Plant-produced vaccine
Plants - genetics
Plants - metabolism
Primates
Recombinant Proteins - chemistry
Tropical bacterial diseases
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Yersinia pestis
Yersinia pestis - immunology
Yersinia pestis - metabolism
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Summary:Abstract Production of vaccine antigens in plants has received considerable attention over the last decade. However, despite many antigens being expressed in plant systems, and promising efficacy data with rodent models, few vaccine candidates have advanced into studies in non-human primates or human clinical trials. Here, we report on the transient expression of the F1 and LcrV antigens of Yersinia pestis in Nicotiana benthamiana . The antigens were expressed as fusions to the thermostable enzyme of Clostridium thermocellum . When administered to Cynomolgus Macaques the purified plant-produced antigens induced serum IgG and IgA responses specific to F1 and LcrV, and conferred complete protection against lethal challenge with Y. pestis . This study clearly demonstrates the efficacy of a plant-produced plague vaccine candidate in a primate model.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.01.017