Collateral projections from the subfornical organ to the median preoptic nucleus and paraventricular hypothalamic nucleus in the rat

Abstract It is morphologically demonstrated that the subfornical organ (SFO) projects to the paraventricular hypothalamic nucleus (PVN) and also projects to the nucleus preopticus medianus (POMe), a relay nucleus of indirect projections from the SFO to PVN. However, it remains unknown, whether or no...

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Bibliographic Details
Published in:Brain research 2008-03, Vol.1198, p.68-72
Main Authors: Duan, Ping-Guo, Kawano, Hitoshi, Masuko, Sadahiko
Format: Article
Language:English
Subjects:
Animals
Axons - physiology
Axons - ultrastructure
Brain Mapping
Cardiovascular Physiological Phenomena
Double label
Fast Blue
Fluorescent Dyes
FluoSpheres
Homeostasis - physiology
Male
Microspheres
Neural Pathways - anatomy & histology
Neural Pathways - physiology
Neurology
Neuronal connection
Neurons - cytology
Neurons - physiology
Paraventricular Hypothalamic Nucleus - anatomy & histology
Preoptic Area - anatomy & histology
Rats
Rats, Sprague-Dawley
Retrograde tracing
Subfornical Organ - anatomy & histology
Water-Electrolyte Balance - physiology
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Summary:Abstract It is morphologically demonstrated that the subfornical organ (SFO) projects to the paraventricular hypothalamic nucleus (PVN) and also projects to the nucleus preopticus medianus (POMe), a relay nucleus of indirect projections from the SFO to PVN. However, it remains unknown, whether or not SFO neurons project collaterally to the POMe and PVN. To confirm this, a double retrograde labeling method was performed on rats using two fluorescent tracers. One tracer (red-colored FluoSpheres: FSR) was injected into the POMe and the other (Fast Blue: FB) was injected into the unilateral PVN at the same time. As a result, many retrogradely labeled neurons were found in the entire SFO. Of these, some neurons showed both FSR and FB fluorescence. Double-labeled neurons were found in about 8.7% of FSR-labeled neurons and 15.5% of FB-labeled neurons. The existence of double-labeled neurons indicates that single neurons in the SFO project simultaneously to the POMe and PVN via collateral axon branches. The data suggest that there are complicated neuronal pathways originating from the SFO in regulating cardiovascular and body fluid homeostasis.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2008.01.035