Clinical usefulness of serum EBV DNA levels of BamHI W and LMP1 for Nasal NK/T-cell lymphoma

Quantitative real‐time polymerase chain reaction (PCR) was utilized to measure serum EBV DNA levels of BamHI W fragment and latent membrane protein 1 (LMP1) in 20 nasal natural killer (NK)/T‐cell lymphoma patients. Both serum EBV DNAs were detected at high levels in all patients, but the levels were...

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Bibliographic Details
Published in:Journal of medical virology 2007-05, Vol.79 (5), p.562-572
Main Authors: Ishii, Hideyuki, Ogino, Takeshi, Berger, Christoph, Köchli-Schmitz, Nicole, Nagato, Toshihiro, Takahara, Miki, Nadal, David, Harabuchi, Yasuaki
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, Viral
BamHI W
Biological and medical sciences
Biomarkers - blood
Carcinogenesis, carcinogens and anticarcinogens
Deoxyribonuclease BamHI - genetics
DNA Primers
DNA, Viral - blood
Epstein-Barr virus
Female
Fundamental and applied biological sciences. Psychology
Herpesvirus 4, Human - genetics
Herpesvirus 4, Human - isolation & purification
Human viral diseases
Humans
Infectious diseases
Killer Cells, Natural
LMP1
Lymphoma, T-Cell - blood
Lymphoma, T-Cell - diagnosis
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
nasal NK/T-cell lymphoma
Nose Neoplasms - blood
Nose Neoplasms - diagnosis
Peptide Fragments - genetics
Polymerase Chain Reaction - methods
Prognosis
quantitative real-time polymerase chain reaction
Tumors
Viral diseases
Viral Matrix Proteins - genetics
Viral Proteins - genetics
Virology
Viruses
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Summary:Quantitative real‐time polymerase chain reaction (PCR) was utilized to measure serum EBV DNA levels of BamHI W fragment and latent membrane protein 1 (LMP1) in 20 nasal natural killer (NK)/T‐cell lymphoma patients. Both serum EBV DNAs were detected at high levels in all patients, but the levels were below the limit of detection in all healthy controls. The BamHI Z fragment, Epstein–Barr‐replication activator (ZEBRA) expression was detected in a small proportion (0.1–3%) of lymphoma cells from 10 (50%) of the patients. Patients with ZEBRA expression showed significantly higher DNA levels of BamHI W and LMP1 (P = 0.0081, P = 0.004), suggesting that EBV DNA may be caused by EBV replication from lymphoma cells. Kaplan–Meier and univariate analyses revealed that high DNA levels of BamHI W and LMP1 at pre‐treatment and high BamHI W DNA level at post‐treatment were associated with short disease‐free survival and overall survival (P 
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.20853