Dietary supplementation with cacao liquor proanthocyanidins prevents elevation of blood glucose levels in diabetic obese mice

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2007-04, Vol.23 (4), p.351-355
• Main Authors: Tomaru, Makoto, D.D.S, Takano, Hirohisa, M.D., Ph.D, Osakabe, Naomi, Ph.D, Yasuda, Akiko, M.S, Inoue, Ken-ichiro, M.D., Ph.D, Yanagisawa, Rie, Ph.D, Ohwatari, Tsuneto, D.D.S., Ph.D, Uematsu, Hiroshi, D.D.S., Ph.D
• Format: Article
• Language: English
• Subjects: Age; Aging - physiology; animal disease models; Animals; antioxidant activity; antioxidants; Antioxidation; Biological and medical sciences; Blood; blood glucose; Blood Glucose - drug effects; Blood Glucose - metabolism; Body Weight - drug effects; Cacao - chemistry; Cacao liquor proanthocyanidins; Chocolate; Cocoa; cocoa powder; Developed countries; Diabetes; Diabetes Mellitus, Experimental - prevention & control; Diet; Dietary Supplements; Dose-Response Relationship, Drug; Enzymes; Feeding. Feeding behavior; Food; food intake; Fructosamine - pharmacology; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; Glucose; glucose tolerance; glycemic effect; Hyperglycemia; Hyperglycemia - prevention & control; Hypertension; Hypoglycemic Agents - pharmacology; Liquor; Mice; Mice, Inbred C57BL; Mice, Obese; noninsulin-dependent diabetes mellitus; Nutrition research; Obesity; Obesity - complications; overweight; Oxidative stress; Pathogenesis; Polyphenols; Postprandial Period; proanthocyanidins; Proanthocyanidins - pharmacology; Rodents; Type 2 diabetes mellitus; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2007.01.007

Abstract Objective Effective approaches should be established to prevent the onset of type 2 diabetes mellitus, which has been increasing in developed countries. The present study examined whether dietary supplementation with cacao liquor proanthocyanidins (CLPr) could prevent elevation of blood glucose levels in mice with diabetes mellitus and obesity. Methods C57BL/KsJ-db/db (db/db) diabetic obese mice and C57BL/KsJ-db/+m (db/+m) control mice were fed a diet containing 0% w/w CLPr (0% CLPr), 0.5% w/w CLPr (0.5% CLPr), or 1.0% w/w CLPr (1.0% CLPr) from age 3 wk to age 6 wk. Levels of blood glucose were measured at 4 and 5 wk of age. The animals were sacrificed and the levels of blood glucose and fructosamine were measured at 6 wk of age. Results The levels of blood glucose and fructosamine were higher in the db/db mice than in the db/+m mice fed a diet containing 0%, 0.5%, or 1.0% CLPr. In the db/+m mice, the levels of blood glucose or fructosamine were not significantly different across animals fed 0% CLPr, 0.5% CLPr, and 1.0% CLPr. In the db/db mice, however, a diet containing 0.5% or 1.0% CLPr decreased the levels of blood glucose and fructosamine compared with that containing 0% CLPr without significant effects on body weights or food consumption. Conclusion Dietary supplementation with CLPr can dose-dependently prevent the development of hyperglycemia in diabetic obese mice. The dietary intake of food or drinks produced from cacao beans might be beneficial in preventing the onset of type 2 diabetes mellitus.