Relationship between human herpesvirus 8 loads and disease stage in classic Kaposi sarcoma patients

Abstract Human herpesvirus 8 (HHV-8), also known as Kaposi sarcoma (KS)-associated herpesvirus, is causally implicated in all forms of KS, including the classic form. Our objective was to investigate the relationship between HHV-8 load in peripheral blood mononuclear cells (PBMCs) and the stage of t...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2007-04, Vol.57 (4), p.387-392
Main Authors: Guttman-Yassky, Emma, Abada, Rinat, Kra-Oz, Zipi, Sattinger, Judith, Perelman, Alex, Bergman, Reuven, Sarid, Ronit
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Classic Kaposi sarcoma, CKS
DNA, Viral - blood
Female
Fundamental and applied biological sciences. Psychology
Herpesvirus 8, Human - genetics
Herpesvirus 8, Human - isolation & purification
Herpesvirus 8, Human - pathogenicity
Human herpesvirus 8
Human herpesvirus 8, HHV-8
Humans
Infectious Disease
Infectious diseases
Internal Medicine
Kaposi sarcoma, KS
Kaposi sarcoma-associated herpesvirus (KSHV)
Leukocytes, Mononuclear - virology
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Polymerase Chain Reaction - methods
Sarcoma, Kaposi - physiopathology
Sarcoma, Kaposi - virology
Skin Neoplasms - physiopathology
Skin Neoplasms - virology
Tumors
Viral Load
Virology
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Summary:Abstract Human herpesvirus 8 (HHV-8), also known as Kaposi sarcoma (KS)-associated herpesvirus, is causally implicated in all forms of KS, including the classic form. Our objective was to investigate the relationship between HHV-8 load in peripheral blood mononuclear cells (PBMCs) and the stage of the disease in classic KS (CKS) patients. HHV-8 loads were measured in 41 PBMC samples from CKS patients with different Krigel-based classification stages using a quantitative real-time polymerase chain reaction assay. Low HHV-8 DNA loads reaching a maximum of 75.5 copies/105 cells were detected in 73.2% of the patients. HHV-8 loads in patients with stages I and II were similarly distributed. An increased detection rate of HHV-8 DNA, although not statistically significant, was evident in patients diagnosed with CKS stages III and IV. We conclude that the measurements of HHV-8 load in PBMCs provide a limited correlation with the clinical stage of KS.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2006.09.012