Baseline microbiota activity and initial bifidobacteria counts influence responses to prebiotic dosing in healthy subjects

Summary Background  Dietary intervention with prebiotics can cause changes in the colonic microbiota and their metabolic activities. Aim  To investigate whether the response to prebiotic dosing is influenced by the baseline metabolic activity of the colonic flora and bifidobacteria counts. Methods ...

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Bibliographic Details
Published in:Alimentary pharmacology & therapeutics 2008-03, Vol.27 (6), p.504-513
Main Authors: DE PRETER, V., VANHOUTTE, T., HUYS, G., SWINGS, J., RUTGEERTS, P., VERBEKE, K.
Format: Article
Language:English
Subjects:
Adult
Bifidobacterium - drug effects
Bifidobacterium - growth & development
Biological and medical sciences
Colon - microbiology
Colony Count, Microbial
Dietary Carbohydrates - administration & dosage
Dietary Carbohydrates - metabolism
Dietary Carbohydrates - pharmacology
Digestive system
Feces - microbiology
Female
Gastroenterology. Liver. Pancreas. Abdomen
Gastrointestinal Transit
Humans
Male
Medical sciences
Pharmacology. Drug treatments
Probiotics - administration & dosage
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Summary:Summary Background  Dietary intervention with prebiotics can cause changes in the colonic microbiota and their metabolic activities. Aim  To investigate whether the response to prebiotic dosing is influenced by the baseline metabolic activity of the colonic flora and bifidobacteria counts. Methods  The 4‐week effect of lactulose (10 g bid.; n = 29) and oligofructose‐enriched inulin (10 g bid.; n = 19) was evaluated in healthy human volunteers. Lactose‐[15N, 15N]‐ureide was used to study the colonic NH3‐metabolism. Urine (48 h) and faeces (72 h) were collected and analysed for p‐cresol and 15N‐content by gas chromatography–mass spectrometry and isotope ratio mass spectrometer, respectively. Faecal bifidobacteria were quantified by real‐time polymerase chain reaction. Results  After the 4‐week prebiotic administration period, the urinary excretion of p‐cresol and 15N was significantly decreased in both groups (P 
ISSN:0269-2813
1365-2036
DOI:10.1111/j.1365-2036.2007.03588.x