Detection of S100 protein from prostatic cancer patients using anti-S100 protein antibody immobilized on POS-PVA discs

• Published in: Biotechnology and bioengineering 2007-05, Vol.97 (1), p.182-187
• Main Authors: Melo-Júnior, Mario R., Araújo Filho, Jorge Luiz S., Cavalcanti, Carmelita L.B., Patu, Vasco José Ramos M., Beltrão, Eduardo I.C., Carvalho Jr, Luiz B.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biological and medical sciences; Biomarkers, Tumor - blood; Biomarkers, Tumor - immunology; Biotechnology; Body fluids; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Gene expression; Humans; immobilization; Immunoglobulins; immunohistochemistry; Male; neoplasm; Neoplasm Proteins - blood; Neoplasm Proteins - immunology; Oncology; Patients; prostate; Prostate cancer; Prostatic Neoplasms - blood; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - immunology; Proteins; Reproducibility of Results; S100 protein; S100 Proteins - blood; S100 Proteins - immunology; Sensitivity and Specificity; Tissues
• ISSN: 0006-3592; 1097-0290
• DOI: 10.1002/bit.21210

The S100 proteins have been extensively used as cancer biomarkers. The objectives of the present work were to immobilize the antibody anti‐protein S100 to a net of semi‐interpenetrated of polysiloxane and polyvinyl alcohol (POS‐PVA discs), to investigate its capacity to capture S100 protein from serum and to quantify it by ELISA in sera from patients with prostatic adenocarcinoma (n = 15) and healthy individuals (n = 10). Also these values were compared to the S100 protein expression in the prostatic tissue through immunohistochemistry. The POS‐PVA discs fixed about 92.8% of the offered antibody (7.75 µg of antibody per disc). The best values of the immobilized no‐marked antibody anti‐S100 and serum dilution were found to be 10 µg and 1:400, respectively. Optical density (OD) values for the sera of patients (0.425 ± 0.042) with prostatic adenocarcinoma were significantly lower (P