Identification and mapping of open chromatin regions within a 140 kb polygenic locus of human chromosome 19 using E. coli Dam methylase

Using transient expression of the E. coli Dam methylase gene and analysis of the distribution of methylated GATC sites, we studied the distribution of open chromatin regions within a 140 kb long human genome segment in HEK-293 cells. Dam methylated sites were found in gene introns, exons, and interg...

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Bibliographic Details
Published in:Genetica 2007-05, Vol.130 (1), p.83-92
Main Authors: Bulanenkova, Svetlana, Snezhkov, Eugene, Nikolaev, Lev, Sverdlov, Eugene
Format: Article
Language:English
Subjects:
Binding Sites
Cell Extracts - analysis
Cells, Cultured
Chromatin - chemistry
Chromatin structure
Chromosome Mapping - methods
Chromosomes, Human, Pair 19
Dam methylase
Deoxyribonucleic acid
DNA
DNA Methylation
E coli
Escherichia coli
Escherichia coli - enzymology
Functional genomics
Gene regulation
Genetics
Genomics
Humans
Models, Biological
Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics
Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism
Transfection
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Summary:Using transient expression of the E. coli Dam methylase gene and analysis of the distribution of methylated GATC sites, we studied the distribution of open chromatin regions within a 140 kb long human genome segment in HEK-293 cells. Dam methylated sites were found in gene introns, exons, and intergenic regions, and their distribution along DNA was uneven. There were regions of high and low density of Dam methylated GATC sites, presumably corresponding to “open” and “closed” chromatin regions, respectively, and to the functional profile of the genomic locus under study. The Dam methylation profile was also generally in agreement with transcriptional activity of genes in the locus. Moreover, DNA regions accessible to Dam methylase apparently coincided with those hypersensitive to DNase I.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-006-0026-1