Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species

Most recent molecular studies revealed the phylogeny of Greek Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes fro...

Full description

Saved in:
Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2007-05, Vol.147 (1), p.1-10
Main Authors: Valakos, Efstratios D., Kourkouli, Andriani, Skopeliti, Margarita, Pafilis, Panayiotis, Poulakakis, Nikolaos, Voutsas, Ioannis F., Lymberakis, Petros, Simou, Chrisi, Voelter, Wolfgang, Tsitsilonis, Ourania E.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Proliferation
Cell Survival
DNA, Mitochondrial - genetics
Geography
Greece
Lacertidae
Lacertilia
Lizards - classification
Lizards - genetics
Lizards - immunology
Lymphocyte Culture Test, Mixed
Lymphocytes - cytology
Male
mtDNA analysis
Phylogeny
Podarcis
Podarcis sp
Reptile phylogeny
Spleen - cytology
Time Factors
Xenogeneic mixed lymphocyte reaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Most recent molecular studies revealed the phylogeny of Greek Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes from six allopatric Podarcis species encountered in Greece, by assessing two-way mixed lymphocyte reaction (MLR)-induced proliferation. On the basis of stimulation indices (S.I.) as determined from cultures set up from xenogeneic splenocytes coincubated in pairs, we generated a phylogenetic tree, fully consistent with the phylogenetic relationships of Podarcis as determined by parallel analyses based on partial mitochondrial (mt) DNA sequences. Although the exact mechanisms triggering lymphocyte responses in lizard two-way xenogeneic MLR are not fully understood, our results show the potential use of cell-mediated immune responses as an additional approach to mtDNA analysis, for species delimitation within specific lizard taxa.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2006.11.032