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Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species
Most recent molecular studies revealed the phylogeny of Greek Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes fro...
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| Published in: | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2007-05, Vol.147 (1), p.1-10 |
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| cited_by | cdi_FETCH-LOGICAL-c385t-faa0b802ac644b54f0990141ed610a975355d1db71500f94fab704d936d2c7823 |
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| cites | cdi_FETCH-LOGICAL-c385t-faa0b802ac644b54f0990141ed610a975355d1db71500f94fab704d936d2c7823 |
| container_end_page | 10 |
| container_issue | 1 |
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| container_title | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology |
| container_volume | 147 |
| creator | Valakos, Efstratios D. Kourkouli, Andriani Skopeliti, Margarita Pafilis, Panayiotis Poulakakis, Nikolaos Voutsas, Ioannis F. Lymberakis, Petros Simou, Chrisi Voelter, Wolfgang Tsitsilonis, Ourania E. |
| description | Most recent molecular studies revealed the phylogeny of Greek
Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes from six allopatric
Podarcis species encountered in Greece, by assessing two-way mixed lymphocyte reaction (MLR)-induced proliferation. On the basis of stimulation indices (S.I.) as determined from cultures set up from xenogeneic splenocytes coincubated in pairs, we generated a phylogenetic tree, fully consistent with the phylogenetic relationships of
Podarcis as determined by parallel analyses based on partial mitochondrial (mt) DNA sequences. Although the exact mechanisms triggering lymphocyte responses in lizard two-way xenogeneic MLR are not fully understood, our results show the potential use of cell-mediated immune responses as an additional approach to mtDNA analysis, for species delimitation within specific lizard taxa. |
| doi_str_mv | 10.1016/j.cbpb.2006.11.032 |
| format | article |
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Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes from six allopatric
Podarcis species encountered in Greece, by assessing two-way mixed lymphocyte reaction (MLR)-induced proliferation. On the basis of stimulation indices (S.I.) as determined from cultures set up from xenogeneic splenocytes coincubated in pairs, we generated a phylogenetic tree, fully consistent with the phylogenetic relationships of
Podarcis as determined by parallel analyses based on partial mitochondrial (mt) DNA sequences. Although the exact mechanisms triggering lymphocyte responses in lizard two-way xenogeneic MLR are not fully understood, our results show the potential use of cell-mediated immune responses as an additional approach to mtDNA analysis, for species delimitation within specific lizard taxa.</description><identifier>ISSN: 1096-4959</identifier><identifier>EISSN: 1879-1107</identifier><identifier>DOI: 10.1016/j.cbpb.2006.11.032</identifier><identifier>PMID: 17347009</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; Cell Proliferation ; Cell Survival ; DNA, Mitochondrial - genetics ; Geography ; Greece ; Lacertidae ; Lacertilia ; Lizards - classification ; Lizards - genetics ; Lizards - immunology ; Lymphocyte Culture Test, Mixed ; Lymphocytes - cytology ; Male ; mtDNA analysis ; Phylogeny ; Podarcis ; Podarcis sp ; Reptile phylogeny ; Spleen - cytology ; Time Factors ; Xenogeneic mixed lymphocyte reaction</subject><ispartof>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2007-05, Vol.147 (1), p.1-10</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-faa0b802ac644b54f0990141ed610a975355d1db71500f94fab704d936d2c7823</citedby><cites>FETCH-LOGICAL-c385t-faa0b802ac644b54f0990141ed610a975355d1db71500f94fab704d936d2c7823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17347009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valakos, Efstratios D.</creatorcontrib><creatorcontrib>Kourkouli, Andriani</creatorcontrib><creatorcontrib>Skopeliti, Margarita</creatorcontrib><creatorcontrib>Pafilis, Panayiotis</creatorcontrib><creatorcontrib>Poulakakis, Nikolaos</creatorcontrib><creatorcontrib>Voutsas, Ioannis F.</creatorcontrib><creatorcontrib>Lymberakis, Petros</creatorcontrib><creatorcontrib>Simou, Chrisi</creatorcontrib><creatorcontrib>Voelter, Wolfgang</creatorcontrib><creatorcontrib>Tsitsilonis, Ourania E.</creatorcontrib><title>Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species</title><title>Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology</title><addtitle>Comp Biochem Physiol B Biochem Mol Biol</addtitle><description>Most recent molecular studies revealed the phylogeny of Greek
Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes from six allopatric
Podarcis species encountered in Greece, by assessing two-way mixed lymphocyte reaction (MLR)-induced proliferation. On the basis of stimulation indices (S.I.) as determined from cultures set up from xenogeneic splenocytes coincubated in pairs, we generated a phylogenetic tree, fully consistent with the phylogenetic relationships of
Podarcis as determined by parallel analyses based on partial mitochondrial (mt) DNA sequences. Although the exact mechanisms triggering lymphocyte responses in lizard two-way xenogeneic MLR are not fully understood, our results show the potential use of cell-mediated immune responses as an additional approach to mtDNA analysis, for species delimitation within specific lizard taxa.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Proliferation</subject><subject>Cell Survival</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Geography</subject><subject>Greece</subject><subject>Lacertidae</subject><subject>Lacertilia</subject><subject>Lizards - classification</subject><subject>Lizards - genetics</subject><subject>Lizards - immunology</subject><subject>Lymphocyte Culture Test, Mixed</subject><subject>Lymphocytes - cytology</subject><subject>Male</subject><subject>mtDNA analysis</subject><subject>Phylogeny</subject><subject>Podarcis</subject><subject>Podarcis sp</subject><subject>Reptile phylogeny</subject><subject>Spleen - cytology</subject><subject>Time Factors</subject><subject>Xenogeneic mixed lymphocyte reaction</subject><issn>1096-4959</issn><issn>1879-1107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkT2P1TAQRSMEYpeFP0CBXNElzPgjjiUa9LQsSCtBAbXl2JPFjyQOdoK0_548vSfRQTVTnHuLe6rqNUKDgO27Y-P7pW84QNsgNiD4k-oaO21qRNBP9x9MW0ujzFX1opQjgOhQ4PPqCrWQGsBcV8dDmvo4x_mBxWna5jSmh-jdyNwc2JRG8tvoMgtudWxNzJVCpbDlx-PO0Uxr9CzT6NaY5sLSwEqaiN1lop_sawou-1hYWchHKi-rZ4MbC7263Jvq-8fbb4dP9f2Xu8-HD_e1F51a68E56DvgzrdS9koOYAygRAotgjNaCaUChl6jAhiMHFyvQQYj2sC97ri4qd6ee5ecfm1UVjvF4mkc3UxpK1aDkKqV3X9BDooDF2IH-Rn0OZWSabBLjpPLjxbBnlTYoz2psCcVFtHuKvbQm0v71k8U_kYu2-_A-zNA-xi_I2Vb9plmTyFm8qsNKf6r_w8CfJrm</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Valakos, Efstratios D.</creator><creator>Kourkouli, Andriani</creator><creator>Skopeliti, Margarita</creator><creator>Pafilis, Panayiotis</creator><creator>Poulakakis, Nikolaos</creator><creator>Voutsas, Ioannis F.</creator><creator>Lymberakis, Petros</creator><creator>Simou, Chrisi</creator><creator>Voelter, Wolfgang</creator><creator>Tsitsilonis, Ourania E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species</title><author>Valakos, Efstratios D. ; 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Podarcis species, which for years remained elusive, due to discordant data produced from various chromosomal, complement fixation and protein studies. In this report, we analyzed cellular immune responses of spleen-derived lymphocytes from six allopatric
Podarcis species encountered in Greece, by assessing two-way mixed lymphocyte reaction (MLR)-induced proliferation. On the basis of stimulation indices (S.I.) as determined from cultures set up from xenogeneic splenocytes coincubated in pairs, we generated a phylogenetic tree, fully consistent with the phylogenetic relationships of
Podarcis as determined by parallel analyses based on partial mitochondrial (mt) DNA sequences. Although the exact mechanisms triggering lymphocyte responses in lizard two-way xenogeneic MLR are not fully understood, our results show the potential use of cell-mediated immune responses as an additional approach to mtDNA analysis, for species delimitation within specific lizard taxa.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>17347009</pmid><doi>10.1016/j.cbpb.2006.11.032</doi><tpages>10</tpages></addata></record> |
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| ispartof | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2007-05, Vol.147 (1), p.1-10 |
| issn | 1096-4959 1879-1107 |
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| source | Elsevier |
| subjects | Animals Base Sequence Cell Proliferation Cell Survival DNA, Mitochondrial - genetics Geography Greece Lacertidae Lacertilia Lizards - classification Lizards - genetics Lizards - immunology Lymphocyte Culture Test, Mixed Lymphocytes - cytology Male mtDNA analysis Phylogeny Podarcis Podarcis sp Reptile phylogeny Spleen - cytology Time Factors Xenogeneic mixed lymphocyte reaction |
| title | Combining immunological and molecular data to assess phylogenetic relations of some Greek Podarcis species |
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