Rad52 Promotes Postinvasion Steps of Meiotic Double-Strand-Break Repair

During DNA double-strand-break (DSB) repair by recombination, the broken chromosome uses a homologous chromosome as a repair template. Early steps of recombination are well characterized: DSB ends assemble filaments of RecA-family proteins that catalyze homologous pairing and strand-invasion reactio...

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Bibliographic Details
Published in:Molecular cell 2008-02, Vol.29 (4), p.517-524
Main Authors: Lao, Jessica P., Oh, Steve D., Shinohara, Miki, Shinohara, Akira, Hunter, Neil
Format: Article
Language:English
Subjects:
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
DNA
DNA Breaks, Double-Stranded
DNA Damage
DNA Repair
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Humans
Meiosis - genetics
Nucleic Acid Conformation
Rad51 Recombinase - genetics
Rad51 Recombinase - metabolism
Rad52 DNA Repair and Recombination Protein - genetics
Rad52 DNA Repair and Recombination Protein - metabolism
Recombination, Genetic
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
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Summary:During DNA double-strand-break (DSB) repair by recombination, the broken chromosome uses a homologous chromosome as a repair template. Early steps of recombination are well characterized: DSB ends assemble filaments of RecA-family proteins that catalyze homologous pairing and strand-invasion reactions. By contrast, the postinvasion steps of recombination are poorly characterized. Rad52 plays an essential role during early steps of recombination by mediating assembly of a RecA homolog, Rad51, into nucleoprotein filaments. The meiosis-specific RecA-homolog Dmc1 does not show this dependence, however. By exploiting the Rad52 independence of Dmc1, we reveal that Rad52 promotes postinvasion steps of both crossover and noncrossover pathways of meiotic recombination in Saccharomyces cerevisiae. This activity resides in the N-terminal region of Rad52, which can anneal complementary DNA strands, and is independent of its Rad51-assembly function. Our findings show that Rad52 functions in temporally and biochemically distinct reactions and suggest a general annealing mechanism for reuniting DSB ends during recombination.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2007.12.014