Heme Oxygenase-1 Inducing Constituent of Prunella vulgaris in HepG2 Cells

Inducible heme oxygenase (HO)-1 is known to play a major role in the pathogenesis of several diseases, and it protects cells against oxidant-mediated injury. The bioassay-guided fractionation of the EtOH extract of the flowered fruit-spike of Prunella vulgaris L. (Labiatae) yielded two ursane-type t...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2008/03/01, Vol.31(3), pp.531-533
Main Authors: Jeong, Gil-Saeng, An, Ren-Bo, Pae, Hyun-Ock, Oh, Gi-Su, Chung, Hun-Taeg, Kim, Youn-Chul
Format: Article
Language:English
Subjects:
23-hydroxyursolic acid
Blotting, Western
Cell Line, Tumor
Cell Survival - drug effects
Enzyme Induction
Flowers - chemistry
Fruit - chemistry
heme oxygenase-1
Heme Oxygenase-1 - biosynthesis
Hepatocytes - drug effects
Hepatocytes - enzymology
HepG2 cell
Humans
Molecular Structure
Plant Extracts - adverse effects
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Powders
Prunella - chemistry
Prunella vulgaris
Structure-Activity Relationship
ursane-type triterpene
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Summary:Inducible heme oxygenase (HO)-1 is known to play a major role in the pathogenesis of several diseases, and it protects cells against oxidant-mediated injury. The bioassay-guided fractionation of the EtOH extract of the flowered fruit-spike of Prunella vulgaris L. (Labiatae) yielded two ursane-type triterpenes, 3β,23-dihydroxyurs-12-en-28-oic acid (23-hydroxyursolic acid) (1) and 3β-hydroxyurs-12-en-28-oic acid (ursolic acid) (2). Western blotting demonstrated that treatment with compound 1 increased the expression of HO-1 in a dose-dependent manner in human liver-derived HepG2 cells. Investigation of structure-related HO-1 inducing activity suggested that the hydroxyl group at the C-23 position in the ursane skeleton is important for this activity.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.31.531