Comparison of expression profiles induced by dust mite in airway epithelia reveals a common pathway

Airway epithelial cells have shown to be active participants in the defense against pathogens by producing signaling and other regulatory molecules in response to the encounter. In previous manuscripts, we have studied the effect of house dust mite (HDM) extract on both an epithelial cell-line (H292...

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Bibliographic Details
Published in:Allergy (Copenhagen) 2008-04, Vol.63 (4), p.461-467
Main Authors: Vroling, A.B, Jonker, M.J, Breit, T.M, Fokkens, W.J, Drunen, C.M. van
Format: Article
Language:English
Subjects:
airway epithelium
allergen
Allergies
Animals
Antigens, Dermatophagoides - immunology
Arachnids
Biological and medical sciences
Cell Line, Tumor
Cells
Cells, Cultured
chemokine
cytokine
Dermatology
Epithelial Cells - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression Profiling
Genes
Humans
Hypersensitivity - genetics
Hypersensitivity - immunology
Infections
Medical research
Medical sciences
microarray
Nasal Mucosa - cytology
Nasal Mucosa - immunology
Oligonucleotide Array Sequence Analysis
Pyroglyphidae - immunology
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Viruses
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Summary:Airway epithelial cells have shown to be active participants in the defense against pathogens by producing signaling and other regulatory molecules in response to the encounter. In previous manuscripts, we have studied the effect of house dust mite (HDM) extract on both an epithelial cell-line (H292) and primary nasal epithelial cell. When we compare these responses we conclude that the H292 cells more closely resemble nasal epithelium of healthy controls (share 107 probe-sets) than of allergic individuals (share 17 probe-sets). Interestingly, probably because of an absent intraindividual variation between samples, more probe-sets (8280) change expression significantly in H292 than in either healthy (555) or allergic (401) epithelium. A direct comparison of all the responses in these epithelial cells reveals a core-response to HDM of just 29 genes. These genes (CCL20, IL-8, CXCL2, CXCL1, IL-1B, AREG, TNFAIP3, HBEGF, PTGS2, BMP2, LDLR, PLAUR, PLAU, NFKB2, NFKB1, JUN, ATF3, EGR1, NPC1, TICAM1, EPHA2, CTGF, DUSP1, SPRY1, TLR-3, complement factor C3, IVNS1ABP, SerpinB3, and PSAT1) have described links with allergy or inflammation and may even describe the well-established relationship between viral infections and allergic exacerbations or allergy development.
ISSN:0105-4538
1398-9995
DOI:10.1111/j.1398-9995.2007.01621.x