Recombinant basic fibroblast growth factor inhibits the airway hyperresponsiveness, mucus production, and lung inflammation induced by an allergen challenge

• Published in: Journal of allergy and clinical immunology 2007-04, Vol.119 (4), p.831-837
• Main Authors: Jeon, Seong Gyu, BSc, Lee, Chun Geun, MD, Oh, Min-Hee, BSc, Chun, Eun-Young, BSc, Gho, Yong Song, PhD, Cho, Sang-Heon, MD, Kim, Jong-Hoon, PhD, Min, Kyung-Up, MD, Kim, You-Young, MD, Kim, Yoon-Keun, MD, Elias, Jack A., MD
• Format: Article
• Language: English
• Subjects: airway hyperresponsiveness; Allergens - administration & dosage; Allergens - immunology; Allergy and Immunology; Animals; Asthma; Biological and medical sciences; Bronchial Hyperreactivity - pathology; Bronchial Hyperreactivity - physiopathology; Bronchial Hyperreactivity - prevention & control; FGF2; Fibroblast Growth Factor 2 - deficiency; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - therapeutic use; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunopathology; Inflammation Mediators - metabolism; Inflammation Mediators - therapeutic use; Injections, Subcutaneous; Lung - immunology; Lung - metabolism; Lung - pathology; Medical sciences; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; Migration; Morphogenesis; Mucus - secretion; mucus production; Muscular system; Ovalbumin - administration & dosage; Ovalbumin - immunology; Pathogenesis; Recombinant Proteins - genetics; Recombinant Proteins - therapeutic use; Rodents; Smooth muscle; Transforming Growth Factor beta1 - biosynthesis; Transforming Growth Factor beta1 - metabolism; Wound healing
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2006.12.653

Background IL-13 is believed to be a central mediator of asthma, and TGF-β1 is a key downstream mediator in the development of IL-13–mediated asthma phenotypes. Objective To evaluate the biological roles of basic fibroblast growth factor (FGF2) in phenotype expression in transgenic (TG) mice overexpressing lung-specific TGF-β1, and the therapeutic effects of recombinant FGF2 in the development of asthma phenotypes. Methods To evaluate the roles of FGF2 in airway hyperresponsiveness (AHR) expression induced by high levels of TGF-β1, TGF-β1 TG (+) mice were bred with FGF2-deficient mice. To evaluate the therapeutic effects of recombinant FGF2 (rFGF2) in the development of asthma, mice were given 10 μg of rFGF2 subcutaneously once a day, 1 hour before the allergen challenge in an asthma mouse model. AHR was evaluated using noninvasive whole-body plethysmography, mucus production by diastase-resistant periodic acid Schiff (DPAS) staining, and lung inflammation using bronchoalveolar lavage (BAL) cellularity and lung histology. Results AHR decreased in TGF-β1 TG (+) mice and was accompanied by the upregulation of FGF2 mRNA expression in lung tissues, when compared with littermate wild-type control mice. Interestingly, AHR was enhanced markedly in TGF-β1 (+) mice with homozygous FGF2 gene disruption. In an asthma mouse model, AHR, mucus production, and lung inflammation were inhibited markedly by rFGF2 treatment. This inhibition was accompanied by downregulation of the allergen-induced proliferation of T cells from regional lymph nodes. Conclusion FGF2 seems to be a key inhibitor in the development of AHR, and rFGF2 treatment constrains the development of asthma phenotypes.