Time Course of Peripheral Oxidative Stress as Consequence of Global Ischaemic Brain Injury in Rats

Free radicals play an important role in the pathogenesis of brain injury. This study evaluates the potential relationship between ischaemia/reperfusion (I/R)-induced brain injury, peripheral oxidative stress (lymphocyte DNA damage), plasma antioxidant potential and uric acid levels. We observed that...

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Bibliographic Details
Published in:Cellular and molecular neurobiology 2008-05, Vol.28 (3), p.431-441
Main Authors: Sivoňová, Monika, Kaplán, Peter, Ďuračková, Zdenka, Dobrota, Dusan, Drgová, Anna, Tatarková, Zuzana, Pavlíková, Martina, Halašová, Erika, Lehotský, Jan
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Biomedical and Life Sciences
Biomedicine
Brain Injuries - complications
Brain Injuries - pathology
Cell Biology
DNA Damage - physiology
Free Radicals - blood
Free Radicals - metabolism
Lymphocytes - metabolism
Lymphocytes - pathology
Male
Neurobiology
Neurosciences
Original Paper
Oxidative Stress - physiology
Rats
Rats, Wistar
Reperfusion Injury - complications
Reperfusion Injury - pathology
Time Factors
Uric Acid - blood
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Summary:Free radicals play an important role in the pathogenesis of brain injury. This study evaluates the potential relationship between ischaemia/reperfusion (I/R)-induced brain injury, peripheral oxidative stress (lymphocyte DNA damage), plasma antioxidant potential and uric acid levels. We observed that 15 min of ischaemia were sufficient to significantly increase lymphocyte DNA damage that remained elevated at the end of early (3 h) reperfusion and at later (72 h) reperfusion time; this parameter was not significantly increased, when compared to preoperated levels. In parallel, antioxidant potential was elevated after 15 min of ischaemia, remained high at early (3 h) reperfusion and decreased again with longer (72 h) reperfusion. A close association between the plasma antioxidant status and the uric acid content has been confirmed by findings that changes in TRAP values positively correlate with uric acid concentration in rat plasma after ischaemic injury. Moreover, results of in vitro experiments with extra uric acid addition to control plasma have shown that uric acid contributes to a greater part of TRAP values. These results indicate a similar time course of brain I/R-associated oxidative stress and peripheral antioxidant defence status and/or oxidative stress in animal experiments.
ISSN:0272-4340
1573-6830
DOI:10.1007/s10571-007-9246-x