Protective and immunomodulatory effect of Gingyo-san in a murine model of acute lung inflammation

• Published in: Journal of ethnopharmacology 2007-05, Vol.111 (2), p.418-426
• Main Authors: Yeh, Chia-Chou, Lin, Chih-Che, Wang, Shulhn-Der, Hung, Che-Ming, Yeh, Ming-Hsien, Liu, Ching-Ju, Kao, Shung-Te
• Format: Article
• Language: English
• Subjects: Acute Disease; Animals; anti-inflammatory agents; AP-1; Arctium lappa; bioassays; Biological and medical sciences; Campanulaceae; chemical constituents of plants; Disease Models, Animal; Drug Evaluation, Preclinical; Drugs, Chinese Herbal - therapeutic use; edema; ethnobotany; Female; Forsythia suspensa; General pharmacology; Gingyo-san; Glycine max; Glycyrrhiza uralensis; herbal medicines; immunomodulators; inflammation; Inflammatory cytokine; Lamiaceae; Lipopolysaccharide; Lonicera japonica; Lophatherum gracile; Lung - drug effects; Lung - immunology; Lung - pathology; Medical sciences; medicinal plants; medicinal properties; Mentha; Mentha haplocalyx; Mice; Mice, Inbred BALB C; NF-κB; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phragmites australis; plant extracts; Plant Extracts - therapeutic use; Pneumonia - drug therapy; Pneumonia - immunology; Pneumonia - pathology; Poaceae; Ptatycodon grandiflorum; respiratory tract diseases; Schizonepeta tenuifolia
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2006.12.014

To investigate the effects of Gingyo-san (GGS), the traditional Chinese medicinal formula, on the acute lung inflammation induced by LPS in vivo, mice were challenged with intratracheal LPS before treatment with GGS or vehicle. In lung morphology, GGS reduced the infiltration of activated polymorphonuclear neutrophils in the airways, decreased pulmonary edema, reduced nitrosative stress, and improved lung morphology. ELISA or RT-PCR detected the expression of cytokines in BALF and lung tissue. The mechanism of these benefits by treatment with GGS including attenuating expression TNFα, IL-1β, IL-6, KC, MCP-1, MIP-2, iNOS, and activation of nuclear factor (NF-κB and AP-1) in BALF and lung tissue. Particularly, GGS also enhanced the anti-inflammatory cytokine (IL-10) and limited the acute lung inflammation. Therefore, its protection activity against LPS-induced lung inflammatory mediators release might be beneficial in the treatment of endotoxin-associated inflammation.