Determination of Biogenic Amines in Squid and White Prawn by High-Performance Liquid Chromatography with Postcolumn Derivatization

A simple method was developed for the determination of biogenic amines in aquatic food products using a reverse-phase high-performance liquid chromatography with postcolumn automatic o-phthalaldehyde derivatization and fluorescence detection. The linearity, repeatability, and recovery of the method...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2007-04, Vol.55 (8), p.3083-3088
Main Authors: Zhao, Qing-Xi, Xu, Jie, Xue, Chang-Hu, Sheng, Wen-Jing, Gao, Rui-Chang, Xue, Yong, Li, Zhao-Jie
Format: Article
Language:English
Subjects:
Animals
biogenic amines
Biogenic Amines - analysis
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Cold Temperature
Crustacea - chemistry
Decapodiformes - chemistry
Fish and seafood industries
food composition
Food industries
Food Preservation
food storage
Fundamental and applied biological sciences. Psychology
Illes argentinus
Litopenaeus vannamei
Reproducibility of Results
reversed-phase high performance liquid chromatography
shrimp
squid
storage temperature
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Summary:A simple method was developed for the determination of biogenic amines in aquatic food products using a reverse-phase high-performance liquid chromatography with postcolumn automatic o-phthalaldehyde derivatization and fluorescence detection. The linearity, repeatability, and recovery of the method for seven amines (tyramine, putrescine, cadaverine, histamine, agmatine, spermidine, and spermine) were evaluated. This optimized method was applied to detect biogenic amines in squid and white prawn during refrigerated storage. Sensory analysis, pH value, and total volatile base nitrogen value were combined to evaluate the freshness quality of these two raw materials. Agmatine and cadaverine in squid, cadaverine, and putrescine in white prawn were the most obviously changed amines during the storage at two different temperatures, and these biogenic amines could be the effective quality indicators for the freshness of the raw aquatic materials. Keywords: Biogenic amine; HPLC; postcolumn derivatization; squid; white prawn
ISSN:0021-8561
1520-5118
DOI:10.1021/jf062887+