Actin Is Involved in Auxin-Dependent Patterning

Polar transport of auxin has been identified as a central element of pattern formation. The polarity of auxin transport is linked to the cycling of pin-formed proteins, a process that is related to actomyosin-dependent vesicle traffic. To get insight into the role of actin for auxin transport, we us...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 2007-04, Vol.143 (4), p.1695-1704
Main Authors: Maisch, Jan, Nick, Peter
Format: Article
Language:English
Subjects:
Actins
Actins - physiology
Auxins
Bacterial Proteins - genetics
Biological and medical sciences
Bundling
Cell division
Cell growth
Cell Line
Cell lines
Development and Hormone Action
Fundamental and applied biological sciences. Psychology
Growth regulators
Indoleacetic Acids - metabolism
Insulin antibodies
Luminescent Proteins - genetics
Metabolism
Microfilaments
Nicotiana - cytology
Nicotiana - metabolism
Plant cells
Plant physiology and development
Plants
Recombinant Fusion Proteins - genetics
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Summary:Polar transport of auxin has been identified as a central element of pattern formation. The polarity of auxin transport is linked to the cycling of pin-formed proteins, a process that is related to actomyosin-dependent vesicle traffic. To get insight into the role of actin for auxin transport, we used patterned cell division to monitor the polarity of auxin fluxes. We show that cell division in the tobacco (Nicotiana tabacum L. cv Bright-Yellow 2) cell line is partially synchronized and that this synchrony can be perturbed by inhibition of auxin transport by 1-N-naphthylphthalamic acid. To address the role of actin in this synchrony, we induced a bundled configuration of actin by overexpressing mouse talin. The bundling of actin impairs the synchrony of cell division and increases the sensitivity to 1-N-naphthylphthalamic acid. Addition of the polarly transported auxins indole-3-acetic acid and 1-naphthyl acetic acid (but not 2,4-dichlorophenoxyacetic acid) restored both the normal organization of actin and the synchrony of cell division. This study suggests that auxin controls its own transport by changing the state of actin filaments.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.106.094052