Multicommuted Fluorometric Multiparameter Sensor for Simultaneous Determination of Naproxen and Salicylic Acid in Biological Fluids

A combined approach based on solid-phase optosensing and multicommutation principles has been applied to develop a method for the simultaneous analysis of two pharmaceuticals (naproxen and salicylic acid) in biological fluids. The multicommuted flow-through optosensor was based on direct native fluo...

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Bibliographic Details
Published in:Analytical Sciences 2007, Vol.23(4), pp.423-428
Main Authors: GARCÍA-REYES, Juan F., ORTEGA-BARRALES, Pilar, MOLINA-DÍAZ, Antonio
Format: Article
Language:English
Subjects:
Anti-Infective Agents - blood
Anti-Infective Agents - urine
Anti-Inflammatory Agents, Non-Steroidal - blood
Anti-Inflammatory Agents, Non-Steroidal - urine
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Fluorescence
Humans
Naproxen - blood
Naproxen - urine
Salicylic Acid - blood
Salicylic Acid - urine
Silica Gel
Silicon Dioxide - chemistry
Software
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Summary:A combined approach based on solid-phase optosensing and multicommutation principles has been applied to develop a method for the simultaneous analysis of two pharmaceuticals (naproxen and salicylic acid) in biological fluids. The multicommuted flow-through optosensor was based on direct native fluorescence measurements of both analgesics using a non-polar sorbent (C18 silica gel) as a solid sensing zone. The flow system was controlled by Java-written home-made software and designed using three-way solenoid valves for an independent automated manipulation of sample and carrier solutions. Using an optimized sampling time, the method was calibrated in the range of 1 - 25 and 5 - 200 ng mL-1. The obtained detection limits were 0.3 and 1.3 ng mL-1 for naproxen and salicylic acid, respectively, with RSD (%) values of better than 2% for both analytes. The proposed methodology was successfully applied to urine, serum and pharmaceutical preparations. Recovery percentages ranging from 96.1 to 104% were obtained for both analytes.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.23.423